Goat anti-Rat IgM (Chain mu) Antibody (Biotin)

Catalog No. ABIN300968

Product Details

Target: IgM

Binding Specificity: Chain mu

Reactivity: Rat

Host: Goat

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Specificity: Anti-biotin, anti-Goat Serum, Rat IgM and Rat Serum. No reaction was observed against Rat IgA or Rat IgG

Purification: Immunoaffinity purified

Immunogen: Rat IgM mu chain.

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:20000 - 1:100000), IF, IHC, WB

Usage: Immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Rat IgM in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:20000 to 1:100000 of the concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody