Chicken anti-Rat IgG (Whole Molecule) Antibody (Alkaline Phosphatase (AP))
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- Target See all IgG products
- IgG
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Binding Specificity
- Whole Molecule
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Reactivity
- Rat
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Host
- Chicken
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Clonality
- Polyclonal
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Conjugate
- Alkaline Phosphatase (AP)
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Application
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Specificity
- Anti-Alkaline Phosphatase (calf intestine), anti-Chicken Serum, Rat IgG and Rat Serum
- Purification
- Immunoaffinity purified
- Immunogen
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Rat IgG whole molecule.
Type of Immunogen: Purified protein
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- Application Notes
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Approved: ELISA (1:1400 - 1:6100), IHC, WB
Usage: Suitable for immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using pNPP (p-nitrophenyl phosphate) catalog no. NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1:1400 to 1:6100 of the stated concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Rat
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.05 M Tris chloride, 0.15 M sodium chloride, 0.001 M magnesium chloride, 0.0001 M zinc chloride, 50 % glycerol, pH 8.0, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze! Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Do not add Sodium azide.
Dilute only prior to immediate use
Each reagent is stable for the period shown on the bottle label if stored as directed. - Storage
- 4 °C
- Storage Comment
- Store at 4°C. Do not freeze.
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
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