Rabbit anti-Human IgG (F(ab')2 Region) Antibody (Biotin)

Catalog No. ABIN301026

Product Details

Target: IgG

Binding Specificity: F(ab')2 Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Specificity: Anti-biotin, anti-Rabbit Serum, Human IgG, Human IgG F(ab')2 and Human Serum. No reaction was observed against Human IgG F(c) or Mouse Serum Proteins

Purification: Immunoaffinity purified

Immunogen: Human IgG F(ab')2 fragment.

Application Details

Application Notes: Approved: ELISA (1:12000 - 1:55000), IF, IHC, WB

Usage: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 μg of Human IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Aliquot to Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C while lyophilized, once reconstituted aliquot and freeze at -20°C.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody