Donkey anti-Human IgG (Whole Molecule) Antibody (HRP)

Catalog No. ABIN301352

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Whole Molecule

Reactivity: Human

Host: Donkey

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Anti-Peroxidase, anti-Donkey Serum, Human IgG and Human Serum. No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c) or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Mouse, Rabbit, Rat and Sheep Serum Proteins

Purification: Immunoaffinity purified

Immunogen: Human IgG whole molecule.

Type of Immunogen: Purified protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:5000 - 1:20000), IHC (1:500 - 1:5000), WB (1:2000 - 1:10000)

Usage: Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-anti body based enzymatic assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: deionized water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % Gentamicin sulfate.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody