CD69 antibody (CD69) (APC)

Details for Product anti-CD69 Antibody No. ABIN301987, Supplier: Log in to see
Antigen
  • 5830438K24Rik
  • AI452015
  • AIM
  • VEA
  • BL-AC/P26
  • CLEC2C
  • EA1
  • GP32/28
  • MLR-3
  • Cd69 molecule
  • CD69 antigen
  • CD69 molecule
  • Cd69
  • CD69
Reactivity
Human
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189
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1
Host
Mouse
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109
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18
4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD69 antibody is conjugated to APC
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43
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2
1
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1
Application
Flow Cytometry (FACS), Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
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93
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23
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Options
Supplier
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Supplier Product No.
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Immunogen anti-µ,-stimulated human B lymphocytes
Clone FN50
Isotype IgG1
Specificity The antibody FN50 recognizes CD69, an lymphocyte early activation marker.
HLDA IV, WS Code A 91
Characteristics The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.
Alternative Name CD69 (CD69 Antibody Abstract)
Background CD69 (C-type lectin domain family 2 C, CLEC2C, also known as AIM) is one of the earliest inducible cell surface molecules acquired during leukocyte activation. This glycoprotein serves as a lectin-type receptor in lymphocytes, NK cells and platelets, it is involved in lymphocyte proliferation. CD69 expression is counteracted on T cells in the AIDS stage of HIV infection, and may be also predictive for clinical response to chemoimmunotherapy.
Research Area CD Antigens, Surface Receptors of Immune Cells, Immunology
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 10 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (1 ml) is sufficient for 100 tests.
Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Flow Cytometry (FACS) image for anti-CD69 (CD69) antibody (APC) (ABIN301987) Surface staining of human peripheral blood using anti-CD69 antibody (clone FN50) afte...
Product cited in: Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).

Drbal, Moertelmaier, Holzhauser, Muhammad, Fuertbauer, Howorka, Hinterberger, Stockinger, Schütz: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Tomescu, Chehimi, Maino, Montaner: "NK cell lysis of HIV-1-infected autologous CD4 primary T cells: requirement for IFN-mediated NK activation by plasmacytoid dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 4, pp. 2097-104, 2007 (PubMed).

Background publications Pitsios, Dimitrakopoulou, Tsalimalma, Kordossis, Choremi-Papadopoulou: "Expression of CD69 on T-cell subsets in HIV-1 disease." in: Scandinavian journal of clinical and laboratory investigation, Vol. 68, Issue 3, pp. 233-41, 2008 (PubMed).

Konjevi?, Jovi?, Vuleti?, Radulovi?, Jeli?, Spuzi?: "CD69 on CD56+ NK cells and response to chemoimmunotherapy in metastatic melanoma." in: European journal of clinical investigation, Vol. 37, Issue 11, pp. 887-96, 2007 (PubMed).