Lysosomal-Associated Membrane Protein 1 (LAMP1) antibody

Antigen: Lysosomal-Associated Membrane Protein 1 (LAMP1)

Synonyms: LAMPA, CD107a, LGP120

Clonality: Monoclonal (B-T47)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS)

Catalog no.: ABIN301994

Additional Information

Alternative name: CD107a

Immunogen: U937 human Caucasian hystiocytic lymphoma cell line

Format: Azide Free

Isotype: IgG1  (Matching secondary antibodies)

Clone: B-T47

Description: CD107a (lysosome-associated membrane protein-1, LAMP-1), together with LAMP-2, is amajor constituent of lysosomal membrane, 1-2% of total CD107a is found also on theplasma membrane. The LAMP proteins are involved in lysosome biogenesis and arerequired for fusion of lysosomes with phagosomes. Increased CD107a immunoreactivity isobserved in neurones, and in glial cells surrounding senile plaques in Alzheimers diseasecases and is localized mainly in medullary epithelial cells, single macrophages andlymphocytes in acute thymic involution. CD107a is a good marker of mast cell activation.

Specificity: The antibody B-T47 recognizes CD107a, a 100-120 kDa glycoprotein expressed mainly onlysosomal, but also on the plasma membrane.

Application Details

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified by ion exchange chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Publications

Publications: Hrdinka, Dráber, Stepánek et al.: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, 2011 (PubMed).