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alpha Tubulin antibody (Biotin)

TUBA1 Reactivity: Human, Mouse, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Paramecium, Turkey, Eisenia fetida WB, IHC (p), ICC, FACS Host: Mouse Monoclonal TU-01 Biotin
Catalog No. ABIN301997
  • Target See all alpha Tubulin (TUBA1) Antibodies
    alpha Tubulin (TUBA1)
    Reactivity
    • 108
    • 73
    • 67
    • 23
    • 19
    • 16
    • 14
    • 12
    • 8
    • 5
    • 5
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Pig, Saccharomyces cerevisiae, Arabidopsis, Nicotiana tabacum, Paramecium, Turkey, Eisenia fetida
    Host
    • 62
    • 54
    • 8
    • 2
    • 2
    • 1
    • 1
    Mouse
    Clonality
    • 71
    • 56
    • 2
    Monoclonal
    Conjugate
    • 85
    • 10
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This alpha Tubulin antibody is conjugated to Biotin
    Application
    • 102
    • 45
    • 42
    • 42
    • 38
    • 32
    • 27
    • 13
    • 7
    • 6
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Flow Cytometry (FACS)
    Specificity
    The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
    Cross-Reactivity (Details)
    Broad species reactivity
    Purification
    Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.
    Immunogen
    Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
    Clone
    TU-01
    Isotype
    IgG1
    Top Product
    Discover our top product TUBA1 Primary Antibody
  • Application Notes
    Western blotting: Recommended dilution: 1-2 μg/mL, reducing conditions.
    Comment

    The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Avoid prolonged exposure to light.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Lukas, Mazna, Valenta, Doubravska, Pospichalova, Vojtechova, Fafilek, Ivanek, Plachy, Novak, Korinek: "Dazap2 modulates transcription driven by the Wnt effector TCF-4." in: Nucleic acids research, Vol. 37, Issue 9, pp. 3007-20, (2009) (PubMed).

    Smertenko, Blume, Viklický, Dráber: "Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies." in: European journal of cell biology, Vol. 72, Issue 2, pp. 104-12, (1997) (PubMed).

    Smertenko, Blume, Viklický, Opatrný, Dráber: "Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells." in: Planta, Vol. 201, Issue 3, pp. 349-58, (1997) (PubMed).

    Nováková, Dráberová, Schürmann, Czihak, Viklický, Dr-aber: "gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies." in: Cell motility and the cytoskeleton, Vol. 33, Issue 1, pp. 38-51, (1996) (PubMed).

    Linhartová, Dráber, Dráberová, Viklický: "Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins." in: The Biochemical journal, Vol. 288 ( Pt 3), pp. 919-24, (1993) (PubMed).

    Dráber, Dráberová, Viklický: "Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head." in: Histochemistry, Vol. 95, Issue 5, pp. 519-24, (1991) (PubMed).

    Dráber, Dráberová, Linhartová, Viklický: "Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies." in: Journal of cell science, Vol. 92 ( Pt 3), pp. 519-28, (1990) (PubMed).

    Dráber, Dráberová, Zicconi, Sellitto, Viklický, Cappuccinelli: "Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin." in: European journal of cell biology, Vol. 41, Issue 1, pp. 82-8, (1987) (PubMed).

    Grimm, Breitling, Little: "Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1." in: Biochimica et biophysica acta, Vol. 914, Issue 1, pp. 83-8, (1987) (PubMed).

    Viklický, Dráber, Hasek, Bártek: "Production and characterization of a monoclonal antitubulin antibody." in: Cell biology international reports, Vol. 6, Issue 8, pp. 725-31, (1982) (PubMed).

  • Target
    alpha Tubulin (TUBA1)
    Alternative Name
    alpha-tubulin (TUBA1 Products)
    Synonyms
    Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody
    Background
    Tubulin alpha 1,The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity, highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening –, this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.,TUBA
    Gene ID
    7277
    UniProt
    Q71U36
    Pathways
    Microtubule Dynamics
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