Neurofilament Medium Protein antibody (Dyomics 547)

Antigen: Neurofilament Medium Protein

Clonality: Monoclonal (NF-09)

Host: Mouse

Reactivity: Mammalian

Conjugate: Dyomics 547

Catalog no.: ABIN302004

Additional Information

Immunogen: Pellet of porcine brain cold stable proteins after depolymerization of microtubules.

Isotype: IgG2a

Clone: NF-09

Description: Neurofilaments (NFs) are a type of intermediate filament (IF) expressed almost exclusivelyin neuronal cells, and in those cells most prominently in large axons. NFs in mostvertebrates are composed of three different polypeptide chains with different molecularweights neurofilament medium protein (NF-M), high (NF-H) and light protein (NF-L),which share sequence and structural similarity in a coiled-coil core domain, but differ in thelength and sequence of their N-termini and more dramatically of their C-termini which in thecase of NF-M and NF-H form the flexible extensions that link NFs to each other and to otherelements in the cytoplasm. NF-M protein tail-mediated interactions of neurofilaments arecritical for size and cytoskeletal architecture of axons, and are mediated, in part, by thehighly phosphorylated tail domain of this protein. NF-M phosphorylation andO-GlcNAcylation are regulated reciprocally and affect its translocation and filamentformation and function. Antibodies to the various neurofilament subunits are very useful celltype markers since the proteins are among the most abundant of the nervous system, areexpressed only in neurons and are biochemically very stable.

Specificity: The antibody NF-09 reacts with both phosphorylated and non-phosphorylated form ofmedium neurofilament protein (160 kDa) of various species.

Application Details

Application Notes: The antibody is designed for direct immunocytochemistry microscopy. Suggested working dilution is 3 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Concentration: 0.1 mg/ml

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Restrictions: For Research Use only

Images

Western blotting analysis of neurofilament medium protein in porcine brain lysate (reducing conditions) by mouse monoclonal NF-09.