HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG) antibody

Antigen: HLA Class I Histocompatibility Antigen, alpha Chain G (HLAG)

Synonyms: MHC-G

Clonality: Monoclonal (87G)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), ELISA

Catalog no.: ABIN302008

Additional Information

Alternative name: HLA-G

Modification: Fab fragment

Immunogen: HLA-B27 transgenic mice were imunized with H-2 identical murine cells transfected with andexpressing genes encoding HLA-G and human beta2-microglobulin.

Isotype: IgG2a

Clone: 87G

Specificity: The antibody 87G recognizes both membrane-bound and soluble forms of HLA-G (HLA-G1and HLA-G5). HLA-G belongs to the MHC Class I molecules (MHC Class Ib, nonclassical)and it is expressed on the surface of trophoblast cells.The antibody 87G blocks interaction of HLA-G with inhibitory receptors. This antibody does not react with: Mouse, Rat .

Application Details

Application Notes: The reagent is intended for flow cytometry and ELISA.

Concentration: 0.5 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from hybridoma culture supernatant by protein-A affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), preserved by filter sterilization, approx. pH 7.4 Preparation: Fab fragments were prepared by ficin digestion of the IgG followed by protein-A affinitychromatography to remove the remaining intact IgG or Fc fra

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Research Area: Major Histocompatibility Complex (MHC)

Restrictions: For Research Use only

Images

Analysis of cytolytical activity

Publications

Publications: Wang, Qiu, Wang et al.: "Role of HLA-G and NCR in Protection of Umbilical Cord Blood Hematopoietic Stem Cells from NK Cell-mediated Cytotoxicity." in: Journal of cellular and molecular medicine, 2010 (PubMed).