LIME antibody (PE)
| Antigen |
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| Clonality |
Monoclonal (LIME-06) |
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Host
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Reactivity
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Conjugate
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PE
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Application
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| Catalog no. |
ABIN302031 |
| Quantity |
0.1 mg (0.1 mg/ml) |
| Price |
533.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 3 to 5 Business Days |
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Immunogen
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Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME.
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Isotype
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IgG1
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Clone
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LIME-06
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Description
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LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusuallybasic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cellcoreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fynkinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylationof C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase ofenzymatic activity compared with the total pool of Lck, thus, LIME serves as a positiveregulator of TCR-dependent T cell signaling. However, under some circumstances, LIMEmay mediate inhibitory signals.
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Specificity
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The antibody LIME-06 was raised against intracellular fragment corresponding to aa141-295 of human LIME, a 30 kDa Lck-interacting transmembrane adaptor expressed by Tcells.
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Application Notes
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The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 5 µg/ml. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.
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Concentration
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0.1 mg/ml
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Buffer
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The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
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Storage
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Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
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Research Area
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Immunology, Adaptive Immunity, CD Antigens
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Restrictions
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For Research Use only
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Alternatives for antigen "LIME", type "Antibodies"
Alternatives 
