Protein tyrosine Phosphatase, Receptor Type, C (PTPRC) antibody (PerCP)

Antigen: Protein tyrosine Phosphatase, Receptor Type, C (PTPRC)

Synonyms: LCA, LY5, B220, CD45, T200, CD45R, GP180, Lca, RT7, L-CA, PTPRC, si:dkeyp-47c5.1

Clonality: Monoclonal (MEM-28)

Host: Mouse

Reactivity: Human

Conjugate: PerCP

Application: Flow Cytometry (FACS)

Catalog no.: ABIN302052

Additional Information

Alternative name: CD45

Immunogen: Human thymocytes and T lymphocytes.

Isotype: IgG1

Clone: MEM-28

Description: CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphataseubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10%of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocytedevelopment and antigen signaling, serving as an important regulator of Src-family kinases.CD45 protein exists as multiple isoforms as a result of alternative splicing, these isoformsdiffer in their extracellular domains, whereas they share identical transmembrane andcytoplasmic domains. These isoforms differ in their ability to translocate into theglycosphingolipid-enriched membrane domains and their expression depends on cell typeand physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 isimportant in promoting cell survival by modulating integrin-mediated signal transductionpathway and is also involved in DNA fragmentation during apoptosis.

Specificity: The antibody MEM-28 reacts with all alternative forms of human CD45 antigen (LeukocyteCommon Antigen), a 180-220 kDa single chain type I transmembrane protein expressed athigh level on all cells of hematopoietic origin, except erythrocytes and platelets.HLDA III, WS Code NL 833a

Application Details

Application Notes: The reagent is designed for Flow Cytometry analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Storage: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Research Area: Immunology, Adaptive Immunity, Hematopoietic Progenitors, Glia marker, CD Antigens, Surface Receptors of Immune Cells

Restrictions: For Research Use only

Publications

Publications: Ilangumaran, Briol, Hoessli: "CD44 selectively associates with active Src family protein tyrosine kinases Lck and Fyn in glycosphingolipid-rich plasma membrane domains of human peripheral blood lymphocytes." in: Blood, Vol. 91, Issue 10, pp. 3901-8, 1998 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).