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C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (C-Term) (HRP)

Details for Product anti-MYC Antibody No. ABIN302064, Supplier: Log in to see
Antigen
  • AU016757
  • bHLHe39
  • c-myc
  • c-Myc
  • C-MYC
  • cmyc
  • CMYC
  • CMyc
  • LOC100136746
  • mMyc
  • MRTL
  • MYC
  • Myc2
  • MYCC
  • Niard
  • Nird
  • RNCMYC
  • zc-myc
Alternatives
anti-Human C-MYC antibody for Flow Cytometry
Epitope
C-Term
93
66
58
40
36
34
27
25
21
17
14
14
13
13
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, All Species
638
189
151
10
8
6
5
5
4
3
3
3
3
3
2
1
1
1
1
1
Host
Mouse
426
201
16
13
8
1
Clonality (Clone)
Monoclonal ()
Conjugate
This C-MYC antibody is conjugated to HRP
40
32
27
15
14
12
4
4
4
4
4
4
4
4
4
3
1
1
1
Application
Western Blotting (WB)
428
230
199
114
104
92
71
44
41
36
22
11
10
10
7
6
4
4
2
2
2
Supplier
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Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Clone 9-00E-10
Isotype IgG1
Specificity The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Characteristics The purified antibody is conjugated with Horseradish Peroxidase (HRP) of high specific activity and RZ=3.
Alternative Name MYC (MYC Antibody Abstract)
Pathways p53 Signaling, Cell Division Cycle
Application Notes The reagent is designed for Western Blotting analysis. Suggested working dilution is 1: 500. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 0.01 % (w/v) thimerosal and 1 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Preservative Thimerosal (Merthiolate)
Precaution of Use Biohazard Informations: This product contains thimerosal which is hazardous.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Product cited in: Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, 2014

Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008

Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004

Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002

Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002

Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001

Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, 1999

Background publications Hoffman, Amanullah, Shafarenko, Liebermann: "The proto-oncogene c-myc in hematopoietic development and leukemogenesis." in: Oncogene, Vol. 21, Issue 21, pp. 3414-21, 2002

Boxer, Dang: "Translocations involving c-myc and c-myc function." in: Oncogene, Vol. 20, Issue 40, pp. 5595-610, 2001

Prendergast: "Mechanisms of apoptosis by c-Myc." in: Oncogene, Vol. 18, Issue 19, pp. 2967-87, 1999

Dang, Resar, Emison, Kim, Li, Prescott, Wonsey, Zeller: "Function of the c-Myc oncogenic transcription factor." in: Experimental cell research, Vol. 253, Issue 1, pp. 63-77, 1999

Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986

Persson, Hennighausen, Taub, DeGrado, Leder: "Antibodies to human c-myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation." in: Science (New York, N.Y.), Vol. 225, Issue 4663, pp. 687-93, 1984