Linker For Activation of T Cells Family, Member 2 (LAT2) antibody

Antigen: Linker For Activation of T Cells Family, Member 2 (LAT2)

Synonyms: LAB, NTAL, WSCR5, WBSCR5, HSPC046, WBSCR15, LAT2, MGC139435

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB)

Catalog no.: ABIN302075

Additional Information

Alternative name: NTAL / LAB

Immunogen: Recombinant cytoplasmic domain (aa 91-243) of human NTAL.

Format: Purified

Description: NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NKcells, mast cells and macrophages. It is a negative regulator of early stages ofBCR-dependent B cell signaling and serves as a negative regulator also in mast cells.However, in mast cells, NTAL also contributes to some activation processes, partiallyoverlapping with LAT function.

Specificity: The polyclonal antibody recognizes the defined epitope (aa 91-243) of human Non-T cellActivation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDatransmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NKcells and myeloid cells. This antibody does not react with: Mouse.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml, incubation 60 min in RT, or overnight at 4 o C Positive control: RAMOS human lymphoma cell lineTHP-1 human monocytic leukemia cell line. Negative control: JURKAT human T cell leukemia cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1%laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8. 2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate withnon-reducing SDS-PAGE sample buffer. Application note: Non-Reducing conditions. 15% separating SDS-PAGE gel.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from rabbit serum by protein-A afinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Western Blotting