Mouse anti-Human IgG (Fc Region), (Heavy Chain) Antibody

Details for Product No. ABIN302085, Supplier: Log in to see
Antigen
Epitope
Fc Region, Heavy Chain
4681
2537
1878
1065
1006
860
298
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reactivity
Human
2849
2735
2296
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Mouse
6705
4831
1795
890
609
388
91
55
52
21
8
5
5
5
2
1
1
Clonality (Clone)
Monoclonal ()
Application
Immunocytochemistry (ICC), ELISA, Western Blotting (WB)
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Immunogen Fusion protein of human IgG Fc fragment.
Clone EM-07
Isotype IgG1
Specificity The antibody EM-07 reacts with Fc part of human IgG heavy chain and with isolated Fc fragments.
Cross-Reactivity (Details) The antibody EM-07 does not crossreact with IgM.
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95 % (by SDS-PAGE)
Background Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Molecular Weight 150 kDa
Research Area Immunology, Secondary Antibodies
Application Notes Western Blotting:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by WB.
ELISA:
Application note: The peroxidase conjugate of this antibody is suitable for detection of human IgG Fc fragments by ELISA.

Working concentrations should be determined by the investigator.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Supplier Images
Western Blotting (WB) image for Mouse anti-Human IgG (Fc Region), (Heavy Chain) antibody (ABIN302085) IgG κ light chain (1), IgG λ light chain (2) and IgG Fc fragment (3) purifi...
Background publications Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).