GCPII / PSMA antibody

Antigen: GCPII / PSMA

Clonality: Monoclonal (GCP-04)

Host: Mouse

Reactivity: Human, Pig (Porcine), Rat (Rattus)

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Catalog no.: ABIN302100

Additional Information

Immunogen: Recombinant fragment of human GCPII (amino acids 44-750) produced in S2 cells

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: GCP-04

Description: Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidicdipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membraneantigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoproteinexpressed in various tissues. In nervous system GCPII cleaves abundantN-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner,to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic,GCPII contributes to pathological conditions regarding e.g. Alzheimer's disease,Huntington's disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to bean interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamateto folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII,which is present in a number of tissues at low levels, is overexpressed in neovasculature ofmost solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer.Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated atthe N-terminus (PSM') than mRNA for membrane-bound GCPII, and this ratio is reversedupon malignant transformation.

Specificity: The antibody GCP-04 recognizes amino acids 100-104 of extracellular domain ofdenaturated glutamate carboxypeptidase II (PSMA, NAALADase, FOLH1), an approximately95-110 kDa transmembrane glycoprotein.

Application Details

Application Notes: Western Blotting - Recommended dilution: 1 µg/ml. Positive control: LNCaP cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% NP-40). Incubate 30 min on ice. Mix lysate with non-reducing/reducing Laemmli SDS-PAGE samplebuffer. Application note: Both reducing and non-reducing conditions.

Concentration: 1 mg/ml

Purity: > 95% (by SDS-PAGE)

Purification: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Storage: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Restrictions: For Research Use only

Images

Immunostaining of a fragment of human GCPII (aminoacids 44-750) produced in S2 cells on Western blot by GCP-04 monoclonal antibody. Lanes 1, 2, 3 represent 800, 400 and 200 pg of the protein.