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Cyclin D1 antibody

CCND1 Reactivity: Human, Rat WB, ELISA, FACS, IP, IHC (p), ICC, IHC (fro) Host: Mouse Monoclonal CD1-1 unconjugated
Catalog No. ABIN302101
  • Target See all Cyclin D1 (CCND1) Antibodies
    Cyclin D1 (CCND1)
    Reactivity
    • 242
    • 128
    • 123
    • 38
    • 19
    • 19
    • 17
    • 10
    • 9
    • 8
    • 4
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat
    Host
    • 211
    • 55
    • 1
    Mouse
    Clonality
    • 199
    • 66
    Monoclonal
    Conjugate
    • 118
    • 29
    • 21
    • 9
    • 8
    • 7
    • 7
    • 7
    • 7
    • 7
    • 7
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Cyclin D1 antibody is un-conjugated
    Application
    • 203
    • 89
    • 71
    • 65
    • 42
    • 30
    • 27
    • 27
    • 24
    • 21
    • 19
    • 12
    • 6
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    The mouse monoclonal antibody CD1.1 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
    Cross-Reactivity (Details)
    Human, Rat, Other not determined
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Purified cyclin D1 protein
    Clone
    CD1-1
    Isotype
    IgG1
    Top Product
    Discover our top product CCND1 Primary Antibody
  • Application Notes
    Immunohistochemistry (frozen sections): Recommended dilution: 2 μg/mL, positive tissue: colon.
    Western blotting: Recommended dilution: 1 μg/mL.
    Immunocytochemistry: Recommended dilution: 1 μg/mL.
    Immunoprecipitation: Recommended dilution: 1 μg/mL.
    Immunohistochemistry (paraffin sections): Heat treatment, sodium citrate buffer pH 6.0.
    Flow cytometry: Intracellular staining, membrane permeabilization is required.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Do not freeze.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Target
    Cyclin D1 (CCND1)
    Alternative Name
    Cyclin D1 (CCND1 Products)
    Synonyms
    BCL1 antibody, D11S287E antibody, PRAD1 antibody, U21B31 antibody, AI327039 antibody, CycD1 antibody, Cyl-1 antibody, bcl-1 antibody, cD1 antibody, cb161 antibody, cycd1 antibody, etID37810.7 antibody, fb52e01 antibody, fc45c08 antibody, fc83a12 antibody, id:ibd1198 antibody, wu:fb52e01 antibody, wu:fc45c08 antibody, wu:fc83a12 antibody, ccnd1b antibody, prad1 antibody, ccnd1 antibody, ccnd1a antibody, bcl1 antibody, d11s287e antibody, u21b31 antibody, CCND1 antibody, cyclin D1 antibody, cyclin D1 S homeolog antibody, cyclin D1 L homeolog antibody, CCND1 antibody, Ccnd1 antibody, ccnd1 antibody, ccnd1.S antibody, ccnd1.L antibody
    Background
    Cyclin D1,Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.,CYC-D1, CCND1, Bcl-1, PRAD1, U21B31
    Gene ID
    595
    UniProt
    P24385
    Pathways
    PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling
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