Endoplasmic Reticulum Aminopeptidase 2 (ERAP2) antibody

Antigen: Endoplasmic Reticulum Aminopeptidase 2 (ERAP2)

Synonyms: LRAP, L-RAP, FLJ23633, FLJ23701, FLJ23807, ERAP2, DKFZp469K0122

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN302141

Additional Information

Alternative name: ERAP2

Gene ID: 64167

Immunogen: C-QYLKKFSYRNAKND

Format: Affinity-purified

Description: All names and symbols: ERAP2, endoplasmic reticulum aminopeptidase 2, FLJ23633, FLJ23701, FLJ23807, L-RAP, LRAP, leukocyte-derived arginine aminopeptidase. Official Symbol: ERAP2. RefSeq number(s): NP_071745.1

Specificity: Human, Dog, Cow

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Approx. 110kDa band observed in Human Spleen lysates (calculated MW of 110kDa according to NP_071745.1). Recommended concentration: 0.5-1.5µg/ml. An additional band of unknown identity was also consistently observed at 50kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Proteolysis / Ubiquitin, Adaptive Immunity

Restrictions: For Research Use only

Images

ABIN302141 (0.5µg/ml) staining of Spleen lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.