OTU Domain Containing 5 (OTUD5) antibody

Antigen: OTU Domain Containing 5 (OTUD5)

Synonyms: DUBA, MGC104871, DKFZp761A052, Sfc7, AA407879, AI553596, BB114028, DXImx46e, FLJ12550, otud5, MGC154072, zgc:154072, si:bz30i22.1, si:rp71-30i22.1

Clonality: Polyclonal

Host: Goat

Application: ELISA

Catalog no.: ABIN302160

Additional Information

Alternative name: DUBA

Gene ID: 55593, 54644 (mouse), 363452 (rat)

Immunogen: C-RQVRGPSQPRKAS

Format: Affinity-purified

Description: All names and symbols: OTUD5, OTU domain containing 5, DKFZp761A052, DUBA, MGC104871, -. Official Symbol: OTUD5. RefSeq number(s): NP_060072.1

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 80kDa band in lysates of cell lines Jurkat and MOLT4 after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 60.6kDa according to NP_060072.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only