ABCC11 antibody (Internal Region)

Catalog No. ABIN302164

Product Details anti-ABCC11 Antibody

Target: ABCC11 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 11 (ABCC11))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCC11 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Purpose: MRP8 / ABCC11

Sequence: KVVEFDRPEV LRK

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-KVVEFDRPEVLRK, from the internal region (near C Terminus) of the protein sequence according to NP_115972.2, NP_660187.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Breast. Recommended concentration: 3.75 μg/mL.

Western Blot: Approx 125 kDa band observed in Human Testis lysates and in preliminary testing of Breast lysate (calculated MW of 150 kDa according to NP_660187.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentrat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for ABCC11

Target: ABCC11 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 11 (ABCC11))

Alternative Name: ABCC11 (ABCC11 Products)

Synonyms: EWWD antibody, MRP8 antibody, WW antibody, ATP binding cassette subfamily C member 11 antibody, ABCC11 antibody

Background: ABCC11, ATP-binding cassette transporter MRP8, ATP-binding cassette, sub-family C (CFTR/MRP), member 11, EWWD, MRP8, WW, ATP-binding cassette protein C11, ATP-binding cassette, sub-family C, member 11, multi-resistance protein 8

Gene ID: 85320

NCBI Accession: NP_115972, NP_660187