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PCNA antibody (Proliferating Cell Nuclear Antigen) (AA 1-261)

Details for Product anti-PCNA Antibody No. ABIN302209, Supplier: Log in to see
Antigen
  • 53/13
  • CG9193
  • DmPCNA
  • Dmel\\CG9193
  • MUS209
  • Mus209
  • PCNA
  • PCNA/mus209
  • Pcna
  • dPCNA
  • l(2)02448
  • l(2)56F
  • l(2)56Fa
  • l(2)s1534
  • pcna
  • AO090003000846
  • PCNAR
  • Pcna/cyclin
  • cb16
  • fb36g03
  • wu:fa28e03
  • wu:fb36g03
  • pcna-A
Alternatives
anti-Human PCNA antibody for Immunohistochemistry
Epitope
AA 1-261, N-Term
33
26
14
13
13
13
8
7
7
7
5
4
4
4
4
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3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
390
169
141
24
23
17
15
12
10
9
8
8
8
8
6
6
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
Host
Mouse
245
189
7
3
Clonality (Clone)
Monoclonal ()
Conjugate
This PCNA antibody is un-conjugated
16
14
7
5
3
3
3
2
2
2
2
2
2
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
314
146
113
110
83
73
70
30
28
23
7
7
5
3
2
2
1
1
1
1
1
1
1
1
1
Supplier
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Immunogen Recombinant human PCNA (1-261aa) purified from E. coli
Clone K1A3
Isotype IgG3
Characteristics Synonyms: Cyclin, Proliferating Cell Nuclear Antigen
Purification Protein-G affinity chromatography
Alternative Name PCNA (PCNA Antibody Abstract)
Background PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by UV irradiation, growth factors and eventually by neighboring tumors.Synonyms: Cyclin, Proliferating Cell Nuclear Antigen
UniProt P12004
Research Area Cancer, Cell Cycle, Chromatin Binding Proteins, DNA/RNA, Proliferation Markers
Pathways Telomere Maintenance, DNA Damage Repair
Application Notes ELISA. Western blot: 1: 1,000 approx. 1: 3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate-Buffered Saline ( pH 7.4) with 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Western Blotting (WB) image for anti-PCNA antibody (Proliferating Cell Nuclear Antigen) (AA 1-261) (ABIN302209) anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 1-261), (N-Term) antibody
Background publications Moldovan, Pfander, Jentsch: "PCNA, the maestro of the replication fork." in: Cell, Vol. 129, Issue 4, pp. 665-79, 2007 (PubMed).

Matsumoto, Moriuchi, Koji et al.: "Molecular cloning of cDNA coding for rat proliferating cell nuclear antigen (PCNA)/cyclin." in: The EMBO journal, Vol. 6, Issue 3, pp. 637-42, 1987 (PubMed).