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Details for Product No. ABIN302248

Checkpoint Kinase 2 (CHEK2) antibody

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Antigen
Synonyms CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Cds1, Rad53, HUCDS1, Chk2, CHK-2
Reactivity
»Alternatives Human
Host
»Alternatives Mouse
Clonality (Clone) Monoclonal ()
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Pubmed 6 references available
Catalog no. ABIN302248
Quantity 50 µg
Price
478.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 6 to 8 Business Days
Immunogen A synthetic peptide obtained from Human Chk2 protein sequence
Clone 73C175-1-1
Isotype IgG1
Characteristics Synonyms: CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2checkpoint homolog
Purification Protein G Chromatography
Alternative Name CHK2
Background Chk2 (checkpoint kinase 2) is the mammalian homolog of the Saccharomyces cerevisiaeRad53 and is required for the DNA damage and replication checkpoints (1). In response tolow-dose ionizing radiation (IR), which occurs in an ataxia telangiectasia mutated(ATM)-dependent manner, Chk2 can phosphorylate the mitosis-inducing phosphataseCdc25C on an inhibitory site, blocking entry into mitosis, and p53 on a regulatory site,causing G1 arrest (2,3). In human tissues, Chk2 is homogeneously expressed in renewingcell populations such as epidermis or intestine, heterogeneous in conditionally renewingtissues, and absent or at low level in static tissues such as muscle or brain (4). Mutationsin Chk2 inactivates DNA damage checkpoint pathway involving Chk2 in lung cancersuggesting that reduced expression of Chk2 may be responsible for the development oflung cancer (5). The Chk2-/- knockout cells are resistant to DNA damage-inducedapoptosis, defective for p53 stabilization and for induction of p53-dependent transcriptssuch as p21 in response to gamma irradiation (6).
UniProt O96017
Application Notes Western blot: 2 µg/ml. Immunohistochemistry on Paraffin Sections: 10 µg/ml. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20min. Remove from heat and let stand at RT in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min at RT. Recommneded Positive Control: Human Spleen. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date 12 months
Background publications Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Shieh, Ahn, Tamai et al.: "The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites." in: Genes & development, Vol. 14, Issue 3, pp. 289-300, 2000 (PubMed).

Hirao, Kong, Matsuoka et al.: "DNA damage-induced activation of p53 by the checkpoint kinase Chk2." in: Science (New York, N.Y.), Vol. 287, Issue 5459, pp. 1824-7, 2000 (PubMed).

Falck, Mailand, Syljuåsen et al.: "The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis." in: Nature, Vol. 410, Issue 6830, pp. 842-7, 2001 (PubMed).

Lukas, Bartkova, Latella et al.: "DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology." in: Cancer research, Vol. 61, Issue 13, pp. 4990-3, 2001 (PubMed).

Matsuoka, Nakagawa, Masuda et al.: "Reduced expression and impaired kinase activity of a Chk2 mutant identified in human lung cancer." in: Cancer research, Vol. 61, Issue 14, pp. 5362-5, 2001 (PubMed).

Alternatives for antigen "Checkpoint Kinase 2 (CHEK2)", type "Antibodies"
Hosts (259), (69), (1)
Reactivities (319), (144), (143), (55), (37), (36), (12), (4), (3), (1), (1)
Applications (243), (115), (74), (67), (60), (34), (26), (19), (8), (3), (3), (2)
Conjugates (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (6), (1), (1)
Epitopes (36), (33), (25), (24), (19), (13), (11), (8), (7), (6), (5), (4), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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