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Checkpoint Kinase 2 (CHEK2) antibody

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Synonyms CHK-2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Cds1, HUCDS1, Rad53, Chk2, fa66f08, wu:fa66f08, zgc:55865
(419), (160), (160), (8), (8), (6), (5), (4), (1), (1), (1)
(336), (88), (8), (1), (1)
Clonality (Clone)
Monoclonal ()
(7), (7), (6), (6), (5), (5), (5), (5), (5), (5), (5), (5), (1), (1), (1), (1), (1)
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(341), (115), (107), (69), (67), (57), (37), (29), (24), (6), (5), (2), (1)
Pubmed 6 references available
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Quantity 50 μg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen A synthetic peptide obtained from Human Chk2 protein sequence
Clone 73C175-1-1
Isotype IgG1
Specificity This antibody detects Serine/Threonine Protein Kinase CHK2 (CHEK2) (Family: Protein Kinase, Subfamily: CDS1).
Characteristics Synonyms: CHEK2, CHEK-2, CHK-2, RAD53, Cds1, Serine/threonine-protein kinase Chk2, CHK2checkpoint homolog
Purification Protein G Chromatography
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background Chk2 (checkpoint kinase 2) is the mammalian homolog of the Saccharomyces cerevisiae Rad53 and is required for the DNA damage and replication checkpoints (1). In response to low-dose ionizing radiation (IR), which occurs in an ataxia telangiectasia mutated (ATM)-dependent manner, Chk2 can phosphorylate the mitosis-inducing phosphatase Cdc25C on an inhibitory site, blocking entry into mitosis, and p53 on a regulatory site, causing G1 arrest (2,3). In human tissues, Chk2 is homogeneously expressed in renewing cell populations such as epidermis or intestine, heterogeneous in conditionally renewing tissues, and absent or at low level in static tissues such as muscle or brain (4). Mutations in Chk2 inactivates DNA damage checkpoint pathway involving Chk2 in lung cancer suggesting that reduced expression of Chk2 may be responsible for the development of lung cancer (5). The Chk2-/- knockout cells are resistant to DNA damage-induced apoptosis, defective for p53 stabilization and for induction of p53-dependent transcripts such as p21 in response to gamma irradiation (6).Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle
Application Notes Western blot: 2 μg/mL. Immunohistochemistry on Paraffin Sections: 10 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at RT in buffer for 20 min. Rinse in 1x TBS with Tween(TBST) for 1 min at RT. Recommneded Positive Control: Human Spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months
Supplier Images
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) antibody (ABIN302248) anti-Checkpoint Kinase 2 (CHEK2) antibody
Background publications Matsuoka, Nakagawa, Masuda et al.: "Reduced expression and impaired kinase activity of a Chk2 mutant identified in human lung cancer." in: Cancer research, Vol. 61, Issue 14, pp. 5362-5, 2001 (PubMed).

Lukas, Bartkova, Latella et al.: "DNA damage-activated kinase Chk2 is independent of proliferation or differentiation yet correlates with tissue biology." in: Cancer research, Vol. 61, Issue 13, pp. 4990-3, 2001 (PubMed).

Falck, Mailand, Syljuåsen et al.: "The ATM-Chk2-Cdc25A checkpoint pathway guards against radioresistant DNA synthesis." in: Nature, Vol. 410, Issue 6830, pp. 842-7, 2001 (PubMed).

Hirao, Kong, Matsuoka et al.: "DNA damage-induced activation of p53 by the checkpoint kinase Chk2." in: Science (New York, N.Y.), Vol. 287, Issue 5459, pp. 1824-7, 2000 (PubMed).

Shieh, Ahn, Tamai et al.: "The human homologs of checkpoint kinases Chk1 and Cds1 (Chk2) phosphorylate p53 at multiple DNA damage-inducible sites." in: Genes & development, Vol. 14, Issue 3, pp. 289-300, 2000 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Catalog No. ABIN302248
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