GFM1 antibody
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- Target See all GFM1 Antibodies
- GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This GFM1 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- No Cross-Reactivity
- Mouse (Murine), Rat (Rattus)
- Characteristics
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
- Purification
- Protein G affinity chromatography
- Immunogen
- A semi-purified mitochondrial preparation was used as the immunogen for the anti-Mitochondrial antibody.
- Clone
- AE-1
- Isotype
- IgG1 kappa
- Top Product
- Discover our top product GFM1 Primary Antibody
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- Application Notes
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Optimal dilution of the anti-Mitochondrial antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order Ab without BSA,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.25-0.5 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the anti-Mitochondrial antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- GFM1 (G-Elongation Factor, Mitochondrial 1 (GFM1))
- Alternative Name
- Mitochondrial (GFM1 Products)
- Synonyms
- COXPD1 antibody, EFG antibody, EFG1 antibody, EFGM antibody, EGF1 antibody, GFM antibody, hEFG1 antibody, AW545374 antibody, D3Wsu133e antibody, Gfm antibody, EF-G antibody, Efg antibody, G elongation factor mitochondrial 1 antibody, G elongation factor, mitochondrial 1 antibody, GFM1 antibody, Gfm1 antibody
- Background
- This mAb recognizes a 60 kDa antigen associated with the mitochondria in human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in human cells only. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. This mAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells.
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