Mouse anti-Human IgM Antibody

Catalog No. ABIN3024945

Product Details

Target: IgM

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G

Immunogen: Recombinant human IgM was used as the immunogen for this anti-IgM antibody.

Clone: IM260

Isotype: IgG1 kappa

Application Details

Application Notes: Due to differences in protocols and secondaries, the anti-IgM antibody may need to be titered for optimal performance.

1. FFPE staining requires boiling sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the anti-IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background: IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously.  IgM antibodies account for approximately 5 % -10 % of all the antibody in the body.

This mAb is specific for the human IgM heavy chain. It does not cross-react with other immunoglobulin heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. IgM antibody is useful in the identification of certain cancer types. Some tumors express a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.