phosphodiesterase 7B (PDE7B) antibody

Antigen: phosphodiesterase 7B (PDE7B)

Synonyms: MGC88256, bA472E5.1, PDE7B, MGC151559, ba472e5.1

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN302521

Additional Information

Characteristics: Synonyms: Phosphodiesterase 7B, MGC88256, cAMP-specific 3', 5'-cyclic phosphodiesterase 7B,rolipram-insensitive phosphodiesterase type 7

Alternative name: PDE7B

Swiss-Prot: Q9NP56

Immunogen: Synthetic peptide - KLH conjugated, internal domain of humanRemarks: Mouse Identity: 94 %Mouse Similarity: 100 %Rat Identity: 94 %Rat Similarity: 100 %

Cross-Reactivity: Human

Description: Phosphodiesterase 7B, a cAMP-specific phosphodiesterase, has been shown to affectcAMP-mediated neural activity and cAMP metabolism in the brain. PDE7B activity isinhibited by dipyridamole, IBMX, and SCH51866. At least three splice variants have beenisolated for PDE7B from rats and they show tissue specific expression patterns.

Specificity: This antibody detects Phosphodiesterase 7B (PDE7B) at internal domain. Species: Human, Mouse, Rat. Others not tested.

Application Details

Application Notes: Immunohistochemistry on paraffin sections (1.25 - 2.5 ug/ml). Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100C for 20min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1xTBS with Tween (TBST) for 1 min. at room temperature. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.32 mg/ml

Purification: Aff - Purified

Buffer: Phosphate buffered saline containing 0.09% sodium azide

Storage: Store the antibody at 2 °C to 8 °C up to one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Research Area: Signaling, Enzymes, Metabolism

Restrictions: For Research Use only