ODC1 antibody

Catalog No. ABIN3025558

Product Details anti-ODC1 Antibody

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This ODC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Recombinant human protein was used as the immunogen for the ODC antibody.

Clone: ODC1-486

Isotype: IgG2a kappa

Application Details

Application Notes: Optimal dilution of the ODC antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the ODC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for ODC1

Target: ODC1 (Ornithine Decarboxylase 1 (ODC1))

Alternative Name: ODC-1 / Ornithine Decarboxylase 1 (ODC1 Products)

Synonyms: CG8721 antibody, Dmel\\CG8721 antibody, dodc1 antibody, odc antibody, fc54f04 antibody, fi06d08 antibody, CHUNP6922 antibody, wu:fc54f04 antibody, wu:fi06d08 antibody, odc2 antibody, xODC1 antibody, xodc2 antibody, DDBDRAFT_0204020 antibody, DDBDRAFT_0237755 antibody, DDB_0204020 antibody, DDB_0237755 antibody, ODC antibody, odc1 antibody, ODC1 antibody, Odc antibody, odc1-a antibody, Ornithine decarboxylase 1 antibody, ornithine decarboxylase 1 antibody, ornithine decarboxylase antibody, diaminopimelate decarboxylase antibody, Orn/DAP/Arg decarboxylase 2 domain-containing protein antibody, Diaminopimelate decarboxylase antibody, Ornithine decarboxylase antibody, ornithine decarboxylase 1 L homeolog antibody, ornithine decarboxylase, structural 1 antibody, Odc1 antibody, odc1 antibody, ODC1 antibody, odc antibody, RR_RS04895 antibody, ASA_RS06380 antibody, LOC100232922 antibody, odc1.L antibody, odc-1 antibody

Background: Recognizes a 53  kDa protein, identified as the Ornithine Decarboxylase (ODC-1). ODC is the initial and rate-limiting enzyme in the biosynthetic pathway of polyamines and is involved in the conversion of ornithine to putrescine. The biological activity of ODC-1 is rapidly induced in response to virtually all agents known to promote cell proliferation including hormones, drugs, growth factors, mitogens, and tumor promoters. Reportedly, ODC mRNA levels are elevated in lung carcinomas as well as in colon adenomas and carcinomas. ODC activity in colorectal carcinomas is greater than those in adenomas and normal mucosa.