Mouse anti-Human IgM Antibody

Catalog No. ABIN3025949

Product Details

Target: IgM

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purification: Protein G affinity chromatography

Immunogen: Heavy chain human IgM was used as the immunogen for this IgM antibody.

Clone: DA4-4-SA-DA4 or HB57

Isotype: IgG1 kappa

Application Details

Application Notes: The optimal dilution of the IgM antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Prediluted IHC only format : incubate for 30 min at RT (2)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: IgM

Abstract: IgM Products

Target Type: Antibody

Background: Recognizes a protein of 75  kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin €™s lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.