CD3 epsilon antibody
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- Target See all CD3 epsilon (CD3E) Antibodies
- CD3 epsilon (CD3E)
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Reactivity
- Human, Mouse, Pig, Rat, Rabbit, Cow, Horse, Guinea Pig, Dog, Hamster, Sheep, Chicken, Opossum, Primate, Cat, Cryptomys amatus (Mole rat), Elephant, Frog
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CD3 epsilon antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP)
- Characteristics
- This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
- Purification
- Protein A purified CD3e antibody
- Immunogen
- Amino acids ERPPPVPNPDYEP (156-168) were used as the immunogen for this CD3e antibody.
- Isotype
- IgG
- Top Product
- Discover our top product CD3E Primary Antibody
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- Application Notes
- The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD3e antibody to be titered up or down for optimal performance.\. FACS: use the CD3e antibody at 0.5-1 μg/million cells,WB: 0.5-1.0 μg/mL,IP: 1-2 μg/500 μg protein lysate,
- Restrictions
- For Research Use only
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- Concentration
- 0.2 mg/mL
- Buffer
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- Target
- CD3 epsilon (CD3E)
- Alternative Name
- CD3e (CD3E Products)
- Synonyms
- cd3e antibody, CD3E antibody, t3e antibody, tcre antibody, AI504783 antibody, CD3 antibody, CD3epsilon antibody, T3e antibody, T3E antibody, TCRE antibody, CD3e molecule antibody, CD3 antigen, epsilon polypeptide antibody, cd3e antibody, CD3E antibody, Cd3e antibody
- Background
- This antibody recognizes the epsilon-chain of CD3 (CD3e), which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the Tcell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the Tcell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the Tcell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the Tcell surface. CD3 antigen is a highly specific marker for Tcells, and is present in majority of Tcell neoplasms.
- Gene ID
- 916
- Pathways
- TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway
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