NEK2 antibody

Catalog No. ABIN302761

Product Details anti-NEK2 Antibody

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to aa 287-299 of NEK2 protein.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic peptide

Application Details

Application Notes: Immunofluorescence. Western Blot: 1: 500 - 1: 2000. Immunohistochemistry: 2.5 μg/mL. ELISA: 1: 2000 - 1: 10000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Target Details for NEK2

Target: NEK2 (NIMA (Never in Mitosis Gene A)-Related Kinase 2 (NEK2))

Alternative Name: NEK2 (NEK2 Products)

Synonyms: AA617254 antibody, C77054 antibody, HsPK21 antibody, NEK2A antibody, NLK1 antibody, ATNEK2 antibody, NIMA-RELATED KINASE2 antibody, NIMA-related kinase 2 antibody, T9J14.24 antibody, T9J14_24 antibody, NIMA related kinase 2 antibody, NIMA (never in mitosis gene a)-related expressed kinase 2 antibody, NIMA-related kinase 2 antibody, NEK2 antibody, Nek2 antibody

Background: NEK2, also called serine/threonine-protein kinase Nek2NimA-related protein kinase 2, NimA-like protein kinase 1 and HSPK 21, is a protein kinase involved in mitotic regulation that may have a role at the G2-M transition and may also play a role in meiosis. NEK2 has a nuclear localization and accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins.Synonyms: HSPK 21, NLK1, NimA-related protein kinase 2, Serine/threonine-protein kinase Nek2

Gene ID: 4751

NCBI Accession: NP_001191111

UniProt: P51955

Pathways: M Phase