Cullin 1 (CUL1) antibody
| Antigen |
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| Synonyms |
LOC100282292, CUL1, Cullin-1, DKFZp459I053, MGC149834, MGC149835, Cul1, zgc:55747, zgc:77500, wu:fc13a10 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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 Alternatives Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (11), ELISA (6), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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| Catalog no. |
ABIN303082 |
| Quantity |
50 µl (Variants) |
| Price |
390.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 7 to 10 Business Days |
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Characteristics
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Synonyms: Cullin-1, CUL-1, CUL1
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Alternative name
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Cullin 1
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Swiss-Prot
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Q13616
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Immunogen
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Synthetic peptide
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Cross-Reactivity
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Human, Mouse (Murine)
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Description
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Cullins assemble a potentially large number of ubiquitin ligases by binding to the RINGprotein ROC1 to catalyse polyubiquitination, as well as binding to various specificityfactors to recruit substrates. Cullin 1 is an essential component of the SCF(SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitinationof proteins involved in cell cycle progression, signal transduction and transcription. In theSCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1subunits. Cul1 may also contribute to catalysis through positioning of the substrate andthe ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1,SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interactswith RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated forminteracts with TIP120A/CAND1 and the interaction negatively regulates the association withSKP1 in the SCF complex.
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Specificity
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This antibody reacts to amino acids 742-752 of Cul1 (C-terminus) coupled to KLH. 766-776. Species: Human, Mouse. Others not tested.
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Application Notes
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Immunoprecipitation. Immunohistochemistry on paraffin sections: 1: 500. Western Blot: 1: 500 - 1: 1000. ELISA: 1: 2000 - 1: 10000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
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Storage
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Store the antibody at -20°C. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
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Research Area
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Apoptosis/Necrosis, Proteasome
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Restrictions
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For Research Use only
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Alternatives for antigen "Cullin 1 (CUL1)", type "Antibodies"
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Hosts
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Rabbit (33)
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Reactivities
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Human (30), Mouse (Murine) (24), Rat (Rattus) (18), Cow (Bovine) (1), Dog (Canine) (1), Horse (Equine) (1), Pig (Porcine) (1)
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Applications
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Immunofluorescence (IF) (19), Flow Cytometry (FACS) (18), Western Blotting (WB) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (11), Immunoprecipitation (IP) (11), ELISA (6), Enzyme Immunoassay (EIA) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (IHC) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Epitopes
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C-Term (2), C-Term AA 766-776 (1)
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