MLL2 antibody (AA 4980-5009)

Catalog No. ABIN3031895

Product Details anti-MLL2 Antibody

Target: MLL2 (Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2))

Binding Specificity: AA 4980-5009

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MLL2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Purification: Purified

Immunogen: A portion of amino acids 4980-5009 from the human protein was used as the immunogen for this MLL2 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the MLL2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the MLL2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Target Details for MLL2

Target: MLL2 (Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2))

Alternative Name: MLL2 (MLL2 Products)

Background: The SET domain is a conserved C-terminal domain that characterizes proteins of the MLL family, including MLL2. The MLL SET domain is a histone H3 Lys4 (K4)-specific methyltransferase whose activity is stimulated with acetylated H3 peptides. The gene for MLL2 encodes a 5,262-amino acid protein containing a SET domain, 5 PHD fingers, potential zinc fingers, and a long run of glutamines interrupted by hydrophobic residues (mostly leucine). They also detected an alternatively spliced form encoding 4,957 amino acids and lacking an N-terminal zinc finger and PHD finger. By analysis of rodent/human hybrid cells and analysis of the Genebridge radiation hybrid panel, they mapped the gene to the 12p13.1-qter region. The 12q12-q13 region is involved in duplications and translocations associated with cancer. By database searching, Karlin et al. (2002) identified 192 human protein sequences that have multiple amino acid runs, many of which are associated with disease, including cancer. Karlin et al. (2002) found that a key aspect of 82 of these protein sequences is their role in transcription, translation, and developmental regulation. MLL2 is a striking example of proteins with multiple amino acid runs, with 22 glutamine runsGenes encoding a significant number of long amino acid runs are potentially associated with diseases, such as cancer.

UniProt: O14686

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Warburg Effect