Lysosomal-Associated Membrane Protein 2 (LAMP2) (Center) antibody

Details for Product No. ABIN303278
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Antigen
Synonyms cd107b, lamp-2, lampb, LAMP2, lamp2, DKFZp459J126, LAMP-2, LGP-B, CD107b, Lamp-2, Lamp-2a, Lamp-2b, Lamp-2c, Mac3, LAMPB, LGP110
Epitope
Center
(15), (10), (7), (7), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(127), (116), (25), (19), (13), (13), (7), (2)
Host
Rabbit
(92), (84), (52), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(32), (21), (16), (11), (5), (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Immunofluorescence (IF), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(100), (100), (54), (52), (48), (41), (29), (29), (28), (10), (2), (2), (2), (1), (1)
Pubmed 6 references available
Quantity 50 μg
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Catalog No. ABIN303278
500.50 $
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Immunogen Synthetic Peptide corresponding to 17 amino acid peptide from near the center of human LAMP-2
Specificity This antibody detects CD107b.
Cross-Reactivity (Details) Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
Purification Immunoaffinity Chromatography
Alternative Name CD107b / LAMP2
Background Lysosme associated membrane proteins, or LAMP1 and LAMP2, are major constituents of the lysosomal membrane. The two have closely related structures, with 37 % sequence homology (2). They are both transmembrane glycoproteins that are localized primarily in lysosomes and late endosomes. Newly synthesized molecules are mostly transported from the trans-Golgi network directly to endosomes and then to lysosomes. A second pathway involves the lamps being delivered from the Golgi to the cell surface, and then along the endocytic pathway to the lysosomes. A minor pathway involves transport via the plasma membrane (3). LAMP2 has also been detected at the plasma membrane of cells, as well as in cells that secrete lysosomal hydrolases. A study in the developmental expresses patterns of membrane LAMP2 transcripts indicate a possible involvement of this protein in cell-cell or cellextracellular matrix interaction, and appear to reflect tissue and cell type specific roles of lysosomes during morphogenesis (4). Upon stimulation, a rapid translocation of intracellular LAMPs to the cell membrane is dependent on a carboxylterminal tyrosine based motif (YXXI) (5). This stimulation has also been shown to have an associated release of histamine, leukotriene C 9$) and prostaglandin D 9@), which shows that LAMP1 and LAMP2 are activation markers for normal mast cells (5). They have also been linked to the inflammatory response in that they promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, and therefore possibly the adhesion of PBMC to the site of inflammation (6). LAMP2 has also been shown to be critical for autophagy, in conversion of early autophagic vacuoles to vacuoles which rapidly degrade their content (7).Synonyms: LAMP-2, LAMP-2C, LAMPB, Lysosome-associated membrane glycoprotein 2
Gene ID 3920
NCBI Accession NP_002285
UniProt P13473
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunocytochemistry.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer. Dilute only prior to immediate use.
Supplier Images
anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (Center) antibody anti-Lysosomal-Associated Membrane Protein 2 (LAMP2) (Center) antibody
Background publications Granger, Green, Gabel et al.: "Characterization and cloning of lgp110, a lysosomal membrane glycoprotein from mouse and rat cells." in: The Journal of biological chemistry, Vol. 265, Issue 20, pp. 12036-43, 1990 (PubMed).

Rohrer, Schweizer, Russell et al.: "The targeting of Lamp1 to lysosomes is dependent on the spacing of its cytoplasmic tail tyrosine sorting motif relative to the membrane." in: The Journal of cell biology, Vol. 132, Issue 4, pp. 565-76, 1996 (PubMed).

Kannan, Stewart, Bounds et al.: "Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium." in: Cellular immunology, Vol. 171, Issue 1, pp. 10-9, 1996 (PubMed).

Lichter-Konecki, Moter, Krawisz et al.: "Expression patterns of murine lysosome-associated membrane protein 2 (Lamp-2) transcripts during morphogenesis." in: Differentiation; research in biological diversity, Vol. 65, Issue 1, pp. 43-58, 1999 (PubMed).

Tanaka, Guhde, Suter et al.: "Accumulation of autophagic vacuoles and cardiomyopathy in LAMP-2-deficient mice." in: Nature, Vol. 406, Issue 6798, pp. 902-6, 2000 (PubMed).

Grützkau, Smorodchenko, Lippert et al.: "LAMP-1 and LAMP-2, but not LAMP-3, are reliable markers for activation-induced secretion of human mast cells." in: Cytometry. Part A : the journal of the International Society for Analytical Cytology, Vol. 61, Issue 1, pp. 62-8, 2004 (PubMed).

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