XAF1
Reactivity: Human
WB, ELISA
Host: Mouse
Monoclonal
3E5
unconjugated
Application Notes
Immunohistochemistry on Paraffin Sections (5 μg/mL). Immunohistochemistry (2 μg/mL). Western Blot (0.5-2 μg/mL). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
XAF-1 binds to XIAP, an inhibitor of caspases-3, -7, and -9, and triggers its relocation from the cytosol to the nucleus. Overexpression of XAF-1 results in the neutralization of XIAP's ability to inhibit cell death. XAF-1 is normally expressed in all adult and fetal tissues but was found to be present in very low levels in a variety of cancer cell lines. In contrast, XIAP levels have been shown to be high in a majority of cell lines. Low XAF-1 and high basal expression of XIAP may therefore play a critical role in maintaining survival of cancer cell lines. Both IFN-a2 and IFN-b can induce XAF-1 mRNA in all cells examined but induction of XAF-1 protein (as observed by immunoblot analysis) was seen only in cell lines sensitive to the apoptotic effects of IFNs.Synonyms: BIRC4-binding protein, BIRC4BP, XIAP-associated factor 1, XIAPAF1