Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE) (pThr500) antibody

Antigen: Inhibitor of kappa Light Polypeptide Gene Enhancer in B-Cells, Kinase epsilon (IKBKE)

Synonyms: IKKE, IKKI, IKK-i, KIAA0151, MGC125294, MGC125295, MGC125297, zgc:100844, IKBKE, MGC137373, MGC130907

Epitope: pThr500

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)

Catalog no.: ABIN303327

Additional Information

Characteristics: Synonyms: I kappa-B kinase epsilon, IKK-epsilon, IKK-E, IKKI, KIAA0151, Inducible I kappa-B kinase,IKK-I, Inhibitor of nuclear factor kappa-B kinase subunit epsilon

Alternative name: IKBKE / IKKE

Swiss-Prot: Q14164

Immunogen: IKKE phospho peptide corresponding to a region of the human protein conjugated toKeyhole Limpet Hemocyanin (KLH). AA Sequence: R-S-R-L-R-pT-L-A-E-V-L (aa 496-506).

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Description: Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essentialmediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellularstimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibitNF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of atleast three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulatedmouse macrophage cells, a novel molecule in the IKK complex has been recently identifiedand designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogensand T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mousemacrophage cell lines. This protein has significant sequence homology with kinasedomains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32and Ser36 of IkBa.

Specificity: This antibody recognises IKK Kinase Epsilon.

Application Details

Application Notes: ELISA. Western Blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections (5 µg/ml). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 1.0 mg/ml

Purification: Aff - Purified

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.09%(w/v) Sodium Azide as preservative.

Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C to -70°C forlonger. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Restrictions: For Research Use only