MHC Class I H-2Ld antibody (Biotin)
Reactivity: Mouse
FACS
Host: Mouse
Monoclonal
30-5-7S
Biotin
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- Target
- MHC Class I H-2Ld
-
Reactivity
- Mouse
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Biotin
-
Application
- Flow Cytometry (FACS)
- Cross-Reactivity (Details)
- Species reactivity (tested):Mouse.
- Purification
- Protein G Chromatography.
- Clone
- 30-5-7S
- Isotype
- IgG2a
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-
- Application Notes
-
Flow Cytometry (See Protocol below).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Protocol
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with cell separation medium.
- Restrictions
- For Research Use only
-
- Concentration
- 0.1 mg/mL
- Buffer
- PBS containing 0.02 % Sodium Azide as preservative and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- Target
- MHC Class I H-2Ld
- Background
- The classical MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant Beta2-microglobulin. These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.Synonyms: H-2 class I histocompatibility antigen, H2-L, L-D alpha chain
- Gene ID
- 10090
- UniProt
- P01897
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