Lamin A/C (LMNA) antibody

Antigen: Lamin A/C (LMNA)

Synonyms: FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, CMT2B1, LGMD1B, Dhe, 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam, cb948, fk66d12, wu:fk66d12, lmna-A, MGC127505, LMNA, fpl, idc, lfp, cddc, emd2, fpld, hgps, ldp1, lmn1, lmnc, pro1, cdcd1, cmd1a, cmt2b1, lgmd1b, Lamin-A

Clonality: Monoclonal (JOL2)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Catalog no.: ABIN303591

Additional Information

Alternative name: Lamin A/C

Immunogen: Recombinant human Lamin A. Myeloma/fusion partners: Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP20/Ag4 myeloma cell line

Format: Supernatant

Isotype: IgG1  (Matching secondary antibodies)

Clone: JOL2

Description: Nuclear Lamins form a network of intermediate-type filaments at the nucleoplasmic site of the nuclear membrane. Two main subtypes of nuclear lamins can be distinguished, i.e. A-type Lamins and B-type Lamins. The A-type Lamins comprise a set of three proteins arising from the same gene by alternative splicing, i.e. Lamin A, Lamin C and Lamin Adel 10, while the B-type Lamins include two proteins arising from two distinct genes, i.e. Lamin B1 and Lamin B2. Recent evidence has revealed that mutations in A-type Lamins give rise to a range of rare but dominant genetic disorders, including Emery-Dreifuss muscular dystrophy, dilated cardiomyopathy with conduction-system disease and Dunnigan-type familial partial lipodystrophy. In addition, the expression of A-type Lamins coincides with cell differentiation and as A-type Lamins specifically interact with chromatin, a role in the regulation of differential gene expression has been suggested for A-type Lamins. Also known as: Lamin A/C , CDCD1, CDDC, CMD1A, CMT2B1, EMD2, FPL, FPLD, HGPS, IDC

Specificity: The antibody reacts with both recombinant and native Lamin A and C in humans. It has been shown to bind to an epitope between amino acids 464-572

Synonyms: FPL, IDC, LFP, CDDC, EMD2, FPLD, HGPS, LDP1, LMN1, LMNC, PRO1, CDCD1, CMD1A, CMT2B1, LGMD1B, Dhe, 2459, 74/76, CG6944, D5, Dm, Dm(0), Dm0, Dm1, Dm2, DmelCG6944, DmLamin, Dmo, DmO, Dm[[0]], Dm[[1]], Dm[[2]], Dm[[mit]], Dm[[o]], DM[[O]], jf27, l(2)04643, l(2)25Ec, l(2)gdh-7, l(2)gdh7, l(2)jf27, lam, Lam Dm[[0]], Lam(Dm0), lamDm0, LamDm[[0], lamDm[[0]], LamDm[[0]], lamin, lamin Dm0, lamin Dm[[0]], Lamin Dm[[0]], Lam[[Dm0]], misg, nlam, cb948, fk66d12, wu:fk66d12, lmna-A, MGC127505, LMNA, fpl, idc, lfp, cddc, emd2, fpld, hgps, ldp1, lmn1, lmnc, pro1, cdcd1, cmd1a, cmt2b1, lgmd1b, Lamin-A

Application Details

Application Notes: Optimal antibody dilution should be determined by titration, however as a guideline for western blot up to 1/200, and for immunoprecipitation use neat

Purification: Unpurified

Buffer: Tissue culture supernatant containing 0.2M Tris/HCl pH7.4 and 510% foetal calf serum

Storage: Store at +4°C for one month, or in small aliquots at -20°C for longer

Research Area: Cytoskeleton, Extracellular Matrix

Images

Immunofluorescence staining of Lamin A/C (clone JOL2) and Ki67 on Human dermal fibroblasts

Publications

Publications: Vaughan, Alvarez-Reyes, Bridger et al.: "Both emerin and lamin C depend on lamin A for localization at the nuclear envelope." in: Journal of cell science, Vol. 114, Issue Pt 14, pp. 2577-90, 2001 (PubMed).