Antizyme Inhibitor 1 (AZIN1) antibody

Antigen: Antizyme Inhibitor 1 (AZIN1)

Synonyms: Oazi, Oazin, AI414949, 1700085L02Rik, AZIN1, oazin, wu:fj32d12, OAZIN, MGC81852, MGC89611, MGC143073, DKFZp459G073, azin1, AZI, OAZ, MGC138338, AZ1, Oaz, AZ-1, ODC-Az, Antizyme, ODCAC, OAZI, ODC1L, MGC691, MGC3832, AZS, azi, oaz, MGC75770, OAZ1, oaz1, oaz1S

Clonality: Monoclonal (HI-12)

Host: Mouse

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Catalog no.: ABIN303592

Additional Information

Alternative name: Oazin (Antizyme inhibitor 1)

Immunogen: affinity-purified Antizyme inhibitor from rat liver

Format: Ion exchange chromatography

Isotype: IgG3  (Matching secondary antibodies)

Clone: HI-12

Description: ODC (ornithine decarboxylase) is a key enzyme in polyamine biosynthesis pathway. The degradation of ODC catalyzed by the 26S proteasome is accelerated by antizyme, an ODC inhibitory protein induced poliamine. Antizyme inhibitor 1 (AZI) specifically binds to antizyme with a higher affinity than that of ODC and thus can release active ODC from inactive ODC-antizyme complex. AZI consists of about 450 amino acids and 50 kDa protein. AZI is highly homologous to ODC but retains no enzymatic activity. AZI is a short-living protein that undergoes proteasomal degradation and the degradation is inhibited by interaction with antizyme. AZI is induced in cells and tissues following growth stimulation, is elevated in some tumors. AZI overexpression leads to increased rates of cell proliferation in the rat carcinoma cells and mouse fibroblasts and this effect appears to be partially independent of the ability of AZI to interact with antizyme. It has been also reported that antizyme and AZI localize to centrosome and may play an important role in the regulation of centrosome homeostasis and oncogenesis.

Synonyms: Oazi, Oazin, AI414949, 1700085L02Rik, AZIN1, oazin, wu:fj32d12, OAZIN, MGC81852, MGC89611, MGC143073, DKFZp459G073, azin1, AZI, OAZ, MGC138338, AZ1, Oaz, AZ-1, ODC-Az, Antizyme, ODCAC, OAZI, ODC1L, MGC691, MGC3832, AZS, azi, oaz, MGC75770, OAZ1, oaz1, oaz1S

Application Details

Application Notes: WB: 0.25 ug/mL. ICC 30 ug/mL

Concentration: 0.25 mg/ml

Purification: Ion exchange chromatography

Buffer: PBS [containing 2 % Block Ace as a stabilizer, 0.1 %Proclin as a bacteriostat]

Storage: Store below

Research Area: Enzymes, Metabolism, Proteasome

Restrictions: For Research Use only

Images

Western blot detection of antizyme inhibitor with the monoclonal antibody. Sample: extract of HTC (rat hepatoma) cells (20 ug). Separation: 11% SDS-PAGE. Membrane: Hybond-P. Primary antibody: HI-12 monoclonal antibody at 260 ng/ml. Secondary antibody: horseradish peroxidase-conjugated anti-mouse or rat IgG (Histofine Simple Stain MAX-PO, Nichirei Bioscience) at a dilution of 1:200,000. (Data from Dr. Y. Murakami)

Fluorescent immunocytochemistry of antizyme inhibitor. Cell: HTC cells. Primary antibody: HI-12 monoclonal antibody at 40 ug/ml. Secondary antibody: anti-mouse immunoglobulin conjugated with Alexa 546 (Invitrogen) (red). The nuclei were stained with TO-PRO 3 (Invitrogen) (blue). (Data from Dr. Y. Murakami)