Cytokeratin 5 (KRT5) antibody

Antigen: Cytokeratin 5 (KRT5)

Synonyms: KRT79, CK5, KRT5, K5, Tfip8, Krt2-5, AW146334, 3300001P10Rik, DDD, EBS2, KRT5A

Clonality: Monoclonal (BV8)

Host: Rabbit

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN303595

Additional Information

Alternative name: Cytokeratin 5

Immunogen: A synthetic peptide from the c-terminus of human Cytokeratin 5.

Format: Supernatant

Isotype: IgG  (Matching secondary antibodies)

Clone: BV8

Specificity: This antibody reacts with cytokeratin 5 intermediate filament protein. Basal cells of human epidermis express acidic cytokeratin 14 and basic cytokeratin 5. Cytokeratin 5 is a 58 kD protein, which is closely related to cytokeratin 6. Cytokeratin 5 and 6 are expressed in basal cell epithelioma, basal cells of prostate, urothelium, vagina, squamous cell carcinomas of skin, tongue, epiglottis and rectal-anal region. Cytokeratin 5 can be useful in the distinction of mesotheliomas from most adenocarcinomas.

Application Details

Application Notes: This antibody may be diluted to a titer of 1:50 to 1:100 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Mesothilioma

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as tissue culture supernatant and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Publications: Irvine, McKenna, Jenkinson et al.: "A mutation in the V1 domain of keratin 5 causes epidermolysis bullosa simplex with mottled pigmentation." in: The Journal of investigative dermatology, Vol. 108, Issue 5, pp. 809-10, 1997 (PubMed).