Keratin 20 (KRT20) antibody

Antigen: Keratin 20 (KRT20)

Synonyms: K20, CD20, CK20, KRT21, MGC35423, 9030623C06Rik, Krt21, KRT20, MGC152269

Clonality: Monoclonal (BV12)

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN303599

Additional Information

Alternative name: Cytokeratin 20

Immunogen: A synthetic peptides from the c-terminus of human Cytokeratin 20 protein

Format: Supernatant

Isotype: IgG

Clone: BV12

Description: Cytokeratin 20 is a Type-I keratin, which is expressed primarily in gastric and intestinal epithelium, urothelium, and Merkel-cells. Cytokeratin 20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. Cytokeratin 20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in Cytokeratin 20.

Specificity: Cytokeratin 20 is a Type-I keratin, which is expressed primarily in gastric and intestinal epithelium, urothelium, and Merkel-cells. Cytokeratin 20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. Cytokeratin 20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in Cytokeratin 20.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25 to 1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 1 mM EDTA buffer, pH 8.0 prior to immunostaining. Positive Control: Colon carcinoma

Concentration: 100ug/ml approximately

Purification: Supernatant

Buffer: This antibody is supplied as tissue culture supernatant and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer, Cytoskeleton, Extracellular Matrix

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Publications

Angus, Kiberu, Purvis et al.: "Cytokeratins in cervical dysplasia and neoplasia: a comparative study of immunohistochemical staining using monoclonal antibodies NCL-5D3, CAM 5.2, and PKK1." in: The Journal of pathology, Vol. 155, Issue 1, pp. 71-5, 1988 (PubMed).

Gatter, Abdulaziz, Beverley et al.: "Use of monoclonal antibodies for the histopathological diagnosis of human malignancy." in: Journal of clinical pathology, Vol. 35, Issue 11, pp. 1253-67, 1983 (PubMed).

Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).