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Microphthalmia-Associated Transcription Factor (MITF) antibody
| Antigen | Microphthalmia-Associated Transcription Factor (MITF) |
| Synonyms | MI, WS2A, bHLHe32, Wh, bw, mi, vit, Bhlhe32, Vitiligo, MGC124309, MGC124310, MITF, CMI9, MITF-H, MITF-M, ws2a, mitfa, MITF-A, LOC100313655, mitfb |
| Clonality | Monoclonal (D5) |
| Host |
 Alternatives Mouse |
| Reactivity |
Alternatives Human |
| Application |
Alternatives Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
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2 references available |
| Catalog no. | ABIN303600 |
| Quantity | 0.5 ml (100ug/ml approximately) (Variants) |
| Price | 278.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | Microphthalmia (MiTF) |
| Immunogen | BALB/C mice were injected with N-terminal fragment of human Mi protein. |
| Format | Ig-fraction |
| Isotype | IgG1 (Matching secondary antibodies) |
| Clone | D5 |
| Specificity | This antibody reacts with a 52-56 kD protein known as Microphthalmia (Mi). This antibody reacts with both melanocytic and non-melanocytic isoforms of Mi. Mi is a basic helix-loop-helix-leucin zipper (b-HLH-ZIP) transcription factor implicated in pigmentation, mast cells and bone development. There are two known isoforms of Mi differing by 66 amino acids at the NH2 terminus. |
Application Details
| Application Notes | Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:50 in an ABC method. However, depending on the staining conditions employed, we suggest that final dilution should be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. |
| Concentration | 100ug/ml approximately |
| Buffer | This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative. |
| Storage | Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label. |
| Research Area | Transcription Factors |
| Restrictions | For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union |
Publications
| Publications |
Hemesath, Price, Takemoto et al.: "MAP kinase links the transcription factor Microphthalmia to c-Kit signalling in melanocytes." in: Nature, Vol. 391, Issue 6664, pp. 298-301, 1998 (PubMed).
Weilbaecher, Hershey, Takemoto et al.: "Age-resolving osteopetrosis: a rat model implicating microphthalmia and the related transcription factor TFE3." in: The Journal of experimental medicine, Vol. 187, Issue 5, pp. 775-85, 1998 (PubMed). |
Alternatives
Alternatives for antigen "Microphthalmia-Associated Transcription Factor (MITF)", type "Antibodies"
| Hosts | Rabbit (30), Mouse (20) |
| Reactivities | Human (47), Mouse (Murine) (24), Rat (Rattus) (22), Dog (Canine) (17), Chicken (16), Cow (Bovine) (16), Horse (Equine) (16), Rabbit (16), Pig (Porcine) (1) |
| Applications | Immunofluorescence (IF) (20), Western Blotting (WB) (20), Flow Cytometry (FACS) (18), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (17), Gel Shift (GS) (12), ELISA (4), Immunoprecipitation (IP) (4), ELISA (Detection) (2), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (IHC) (2), Electrophoretic Mobility-Shift Assay (EMSA) (1), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1) |
| Conjugates | Biotin (4), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
