Octamer-Binding Transcription Factor-3 (POU5F1) antibody

Antigen: Octamer-Binding Transcription Factor-3 (POU5F1)

Synonyms: OCT3, OCT4, OTF3, OTF4, MGC22487

Clonality: Monoclonal (NRG1-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Catalog no.: ABIN303602

Additional Information

Alternative name: OCT-4

Immunogen: BALB/C mice injected with synthetic peptide of human OCT-4 origin

Format: Ig-fraction

Isotype: IgG2b  (Matching secondary antibodies)

Clone: NRG1-1

Specificity: OCT-4 (also known as POU5F1), a transcription factor that has been recognized as fundamental in the maintenance of pluripotency in embryonic stem cells and primordial germ cells, has been proposed as a useful marker for Germ Cell Tumors (GCTs) that exhibit features of pluripotentiality, specifically seminoma / dysgerminoma / germinoma and embryonal carcinoma. OCT-4 immunostaining has been shown to be a sensitive and specific marker for seminomatous / (dys)germinomatous tumours and in embryonal carcinoma variants of non-seminomatous GCTs, whether in primary gonadal or extragonadal sites or in metastatic lesions.

Application Details

Application Notes: Titer/Working Dilution: This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. However, depending on the staining conditions employed, we suggest final dilution to be determined by the user. Staining Protocol: We suggest an incubation period of 30 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the end user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Positive Control: Seminoma

Concentration: 100ug/ml approximately

Buffer: This antibody is supplied as purified immunoglobulin fraction containing sodium azide as preservative

Storage: Store the antibody at 2-8o c. Do not use it beyond the expiration date stated on the label.

Research Area: Cancer

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Formalin fixed paraffin embedded human seminomatous tumor stained with OCT-4 antibody

Publications

Publications: Hattab, Tu, Wilson et al.: "OCT4 immunohistochemistry is superior to placental alkaline phosphatase (PLAP) in the diagnosis of central nervous system germinoma." in: The American journal of surgical pathology, Vol. 29, Issue 3, pp. 368-71, 2005 (PubMed).

Lau, Chang: "OCT4: a sensitive and specific immunohistochemical marker for metastatic germ cell tumors." in: Advances in anatomic pathology, Vol. 13, Issue 2, pp. 76-9, 2006 (PubMed).

Sung, Jones, Beck et al.: "OCT4 is superior to CD30 in the diagnosis of metastatic embryonal carcinomas after chemotherapy." in: Human pathology, Vol. 37, Issue 6, pp. 662-7, 2006 (PubMed).

Jung, Chu, Shiu et al.: "Expression of OCT4 in the primary germ cell tumors and thymoma in the mediastinum." in: Applied immunohistochemistry & molecular morphology : AIMM / official publication of the Society for Applied Immunohistochemistry, Vol. 14, Issue 3, pp. 273-5, 2006 (PubMed).

Jones, MacLennan, Bonnin et al.: "Screening for intratubular germ cell neoplasia of the testis using OCT4 immunohistochemistry." in: The American journal of surgical pathology, Vol. 30, Issue 11, pp. 1427-31, 2006 (PubMed).

Cheng, Sung, Cossu-Rocca et al.: "OCT4: biological functions and clinical applications as a marker of germ cell neoplasia." in: The Journal of pathology, Vol. 211, Issue 1, pp. 1-9, 2006 (PubMed).