Biotin antibody (Cy3.5)
| Antigen |
|
| Clonality |
Polyclonal |
|
Host
|
|
|
Conjugate
|
FITC (19), HRP (15), Texas Red (TR) (10), Alkaline Phosphatase (AP) (8), PE (4), Cy2 (3), Biotin (2), Colloidal Gold (15 OD) (2), Cy5.5 (2), Rhodamine (2), TRITC (2), 488-NHS Ester (1), Cy3.5 (1), Cy5 (1), DTAF (1), Peroxidase (POD) (1)
|
| Catalog no. |
ABIN303627 |
| Quantity |
1mg |
| Price |
309.00 $ Plus shipping costs $35.00
|
| Shipping to |
|
| Availability |
Ships within 5 Business Days |
|
Immunogen
|
Biotin conjugated to Keyhole Limpet Hemocyanin (KLH)
|
|
Format
|
|
|
Description
|
Label: Cy3.5 (Cyanine 3.5-OSu) (Molecular Weight 1,286 daltons). Absorption Wavelength: 581 nm Emission Wavelength: 596 nm. Fluorochrome/Protein Ratio: 2.2 moles Cy3.5 per mole of Goat IgG F(ab)2
|
|
Application Notes
|
Suitable for highly specific immunological methods requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
|
|
Purity
|
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Biotin coupled to agarose beads followed by pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, biotin conjugated Goat IgG and biotin conjugated BSA. No reaction was observed against anti-Goat IgG F(c) or anti-Pepsin.
|
|
Buffer
|
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2. Stabilizer: 10 mg/ml Bovine Serum Albumin (BSA) IgG and Protease free. Preservative: 0.01% (w/v) Sodium Azide
|
|
Storage
|
Store vial at 4
|
|
Research Area
|
Signaling, Metabolism, Epitope Tags, Tags/Labels
|
|
Restrictions
|
For Research Use only
|
Alternatives for antigen "Biotin", type "Antibodies"
|
Hosts
|
Goat (81), Rabbit (30), Mouse (27), Sheep (1)
|
|
Reactivities
|
Human (1)
|
|
Applications
|
Western Blotting (WB) (65), ELISA (64), Flow Cytometry (FACS) (47), Immunofluorescence (IF) (34), Immunoprecipitation (IP) (29), Immunohistochemistry (IHC) (24), Immunohistochemistry (Frozen Sections) (IHC (fro)) (18), Enzyme Immunoassay (EIA) (16), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (10), Immunodiffusion (ID) (5), Dot Blot (Dot) (4), Immunocytochemistry (ICC) (3), Immunoelectrophoresis (IEP) (1)
|
|
Conjugates
|
FITC (19), HRP (15), Texas Red (TR) (10), Alkaline Phosphatase (AP) (8), PE (4), Cy2 (3), Biotin (2), Colloidal Gold (15 OD) (2), Cy5.5 (2), Rhodamine (2), TRITC (2), 488-NHS Ester (1), Cy3.5 (1), Cy5 (1), DTAF (1), Peroxidase (POD) (1)
|
Alternatives 
