WNT2B antibody (N-Term)

Catalog No. ABIN3042337

Product Details anti-WNT2B Antibody

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Binding Specificity: AA 87-112, N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT2B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Protein Wnt-2b(WNT2B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequence: QRYPDIMRSV GEGAREWIRE CQHQFR

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protein Wnt-2b(WNT2B) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: wingless-type MMTV integration site family, member 2B
Protein Name: Protein Wnt-2b

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt2b (87-112 aa QRYPDIMRSVGEGAREWIRECQHQFR), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Wnt2b is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for WNT2B

Target: WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))

Alternative Name: WNT2B (WNT2B Products)

Synonyms: WNT13 antibody, XWNT2 antibody, wnt2b antibody, XWnt-2 antibody, Xwnt-2b antibody, wnt-2b antibody, xwnt2b antibody, WNT2B antibody, Wnt13 antibody, Wnt family member 2B antibody, wingless-type MMTV integration site family, member 2Ba antibody, Wnt family member 2B L homeolog antibody, wingless-type MMTV integration site family, member 2B antibody, wingless-type MMTV integration site family, member 2Bb antibody, WNT2B antibody, Wnt2b antibody, wnt2ba antibody, wnt2b.L antibody, wnt2bb antibody

Background: WNT2B (Wingless-Type MMTV Integration Site Family Member 2B), is a protein that in humans is encoded by the WNT2B gene. This gene encodes a member of the wingless-type MMTV integration site (WNT) family of highly conserved, secreted signaling factors. Katoh et al. (1996) used fluorescence in situ hybridization to map the WNT13 gene to chromosome 1p13. WNT family members function in a variety of developmental processes including regulation of cell growth and differentiation and are characterized by a WNT-core domain. This gene may play a role in human development as well as human carcinogenesis.

Synonyms: Protein Wnt-13 antibody|Protein Wnt-2b antibody|Wingless type MMTV integration site family, member 13 antibody|Wingless type MMTV integration site family, member 2B antibody|WNT13 antibody|Wnt2b antibody|WNT2B_HUMAN antibody|XWNT2 antibody|XWNT2, Xenopus, homolog of antibody

Gene ID: 7482

UniProt: Q93097

Pathways: WNT Signaling