WNT1 antibody (N-Term)

Catalog No. ABIN3042350

Product Details anti-WNT1 Antibody

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Binding Specificity: AA 45-74, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WNT1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Proto-oncogene Wnt-1(WNT1) detection. Tested with WB in Human.

Sequence: NLLTDSKSLQ LVLEPSLQLL SRKQRRLIRQ

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Proto-oncogene Wnt-1(WNT1) detection. Tested with WB in Human.
Gene Name: wingless-type MMTV integration site family, member 1
Protein Name: Proto-oncogene Wnt-1

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1 (45-74aa NLLTDSKSLQLVLEPSLQLLSRKQRRLIRQ), identical to the related mouse and rat sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Wnt1 is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-WNT1 antibody (ABIN3042350)

Mi, Li, Zhang, Zhao, Peng, Yang, Zheng: "MicroRNA-139-5p regulates C2C12 cell myogenesis through blocking Wnt/β-catenin signaling pathway." in: Biochemistry and cell biology = Biochimie et biologie cellulaire, Vol. 93, Issue 1, pp. 8-15, (2015) (PubMed).

Dong, Duan, Han, Zhang, Wu: "Suppression of wingless-type MMTV integration site family, member 1 expression by small interfering RNA inhibits U251 glioma cell growth in vitro." in: Oncology letters, Vol. 9, Issue 1, pp. 81-85, (2014) (PubMed).

Target Details for WNT1

Target: WNT1 (Wingless-Type MMTV Integration Site Family, Member 1 (WNT1))

Alternative Name: WNT1 (WNT1 Products)

Synonyms: Xint-1 antibody, Xwnt1 antibody, int-1 antibody, int1 antibody, wnt-1 antibody, wnt1-a antibody, Int-1 antibody, Wnt-1 antibody, sw antibody, swaying antibody, BMND16 antibody, INT1 antibody, OI15 antibody, Int1 antibody, WNT-1 antibody, sb:eu647 antibody, zgc:194464 antibody, zgc:194478 antibody, WNT1 antibody, Wnt family member 1 antibody, Wnt family member 1 L homeolog antibody, wingless-type MMTV integration site family, member 1 antibody, WNT1 antibody, wnt1.L antibody, Wnt1 antibody, wnt1 antibody

Background: Wingless-type MMIV integration site family, member 1 is a protein that in humans is encoded by the WNT1 gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 12q13.12. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. The Wnt1 protein functions in the induction of the mesencephalon and cerebellum. However, the gene mutations might not have a significant role in Joubert syndrome.

Synonyms: BMND16 antibody|INT1 antibody|OI15 antibody|oncogene Int1 antibody|Proto oncogene protein Wnt 1 antibody|Proto-oncogene Int-1 homolog antibody|Proto-oncogene Wnt-1 antibody|Wingless type MMTV integration site family member 1 antibody|wingless-type MMTV integration site family, member 1 (oncogene INT1) antibody|Wnt 1 antibody|wnt1 antibody|WNT1_HUMAN antibody

Gene ID: 7471

UniProt: P04628

Pathways: WNT Signaling, Dopaminergic Neurogenesis