GDNF antibody (AA 78-211)

Catalog No. ABIN3042421

Product Details anti-GDNF Antibody

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Binding Specificity: AA 78-211

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GDNF antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB in Human,Rat.

Cross-Reactivity (Details): Predicted Cross Reactivity: human
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Glial cell line-derived neurotrophic factor(GDNF) detection. Tested with WB in Human,Rat.
Gene Name: glial cell derived neurotrophic factor
Protein Name: Glial cell line-derived neurotrophic factor

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human GDNF recombinant protein (Position: S78-I211). Human GDNF shares 93% amino acid (aa) sequence identity with both mouse and rat GDNF.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, The detection limit for GDNF is approximately 0.25 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-GDNF antibody (ABIN3042421)

A, Xu, Zhao, Li, Qi, Li, Wang, Zhou: "Surface functionalization of TiO2 nanotubes with minocycline and its in vitro biological effects on Schwann cells." in: Biomedical engineering online, Vol. 17, Issue 1, pp. 88, (2018) (PubMed).

Zhang, Shi, Li, Zhang, Hao: "Lipopolysaccharide inhibits the self-renewal of spermatogonial stem cells in vitro via downregulation of GDNF expression in Sertoli cells." in: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 87-93, (2014) (PubMed).

Liu, Wang, Liu, Yang, Xu, Ren: "Antenatal taurine reduces cerebral cell apoptosis in fetal rats with intrauterine growth restriction." in: Neural regeneration research, Vol. 8, Issue 23, pp. 2190-7, (2014) (PubMed).

Li, Xia, Zhang, Wu: "S100B protein, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor in human milk." in: PLoS ONE, Vol. 6, Issue 6, pp. e21663, (2011) (PubMed).

Target Details for GDNF

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF (GDNF Products)

Synonyms: gdnf antibody, GDNF antibody, AI385739 antibody, gdnfa antibody, hfb1-gdnf antibody, ATF1 antibody, ATF2 antibody, HFB1-GDNF antibody, HSCR3 antibody, glial cell derived neurotrophic factor antibody, glial cell derived neurotrophic factor a antibody, glial cell line derived neurotrophic factor antibody, glial cell derived neurotrophic factor L homeolog antibody, Gdnf antibody, gdnfa antibody, GDNF antibody, gdnf.L antibody

Background: Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF is also a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. In addition to its potential role in the differentiation and survival of central nervous system neurons, it has profound effects on kidney organogenesis and the development of the peripheral nervous system. GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons.

Synonyms: gdnf antibody|GDNF_HUMAN antibody|Glial cell derived neurotrophic factor antibody|Glial Cell Line Derived Neurotrophic Factor antibody|Glial cell line-derived neurotrophic factor antibody|Glial derived neurotrophic factor antibody|HFB1 GDNF antibody|hGDNF antibody|HSCR3 antibody

Gene ID: 2668

UniProt: P39905

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration