HGF antibody (N-Term)

Catalog No. ABIN3042443

Product Details anti-HGF Antibody

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Binding Specificity: AA 33-64, N-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HGF antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Hepatocyte growth factor(HGF) detection. Tested with WB in Mouse.

Sequence: QKKRRNTLHE FKKSAKTTLT KEDPLLKIKT KK

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hepatocyte growth factor(HGF) detection. Tested with WB in Mouse.
Gene Name: hepatocyte growth factor (hepapoietin A, scatter factor)
Protein Name: Hepatocyte growth factor

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of mouse HGF (33-64aa QKKRRNTLHEFKKSAKTTLTKEDPLLKIKTKK), different from the related human sequence by five amino acids, and from the related rat sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, The detection limit for HGF is approximately 0.1 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-HGF antibody (ABIN3042443)

Fan, Qu, Chu, Gao, Gao, Chen, Tian: "MicroRNA-210 promotes angiogenesis in acute myocardial infarction." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5658-5665, (2018) (PubMed).

Yu, He, Jiang, He, Fan, Wang, Geng, Dong: "Expression and tissue distribution of hepatocyte growth factor (HGF) and its receptor (c-Met) in alpacas (Vicugna pacos) skins associated with white and brown coat colors." in: Acta histochemica, (2015) (PubMed).

Wen, Zhou, Luo, Zhou, Ma: "Pro-osteogenic effects of fibrin glue in treatment of avascular necrosis of the femoral head in vivo by hepatocyte growth factor-transgenic mesenchymal stem cells." in: Journal of translational medicine, Vol. 12, pp. 114, (2015) (PubMed).

Zhang, Lv, Yang, Han, Zhang, Zhang, Zong, Gao, Li, Zhao, Li, Yang, Yu, Li, Zhang, Wei: "Corticosterone mediates the inhibitory effect of restraint stress on the migration of mesenchymal stem cell to carbon tetrachloride-induced fibrotic liver by downregulating CXCR4/7 expression." in: Stem cells and development, Vol. 24, Issue 5, pp. 587-96, (2015) (PubMed).

Hu, Fang, Qian, Fang, Wang, Wang: "Telmisartan prevents angiotensin II-induced endothelial dysfunction in rabbit aorta via activating HGF/Met system and PPARγ pathway." in: Fundamental & clinical pharmacology, Vol. 28, Issue 5, pp. 501-11, (2014) (PubMed).

Zhang, Wang, Ma, Liu, Zhang, Zhu: "Effect of herba centellae on the expression of HGF and MCP-1." in: Experimental and therapeutic medicine, Vol. 6, Issue 2, pp. 427-434, (2013) (PubMed).

Li, Jiang, Feng et al.: "Isogenic mesenchymal stem cells transplantation improves a rat model of chronic aristolochic acid nephropathy via upregulation of hepatic growth factor and downregulation of transforming growth ..." in: Molecular and cellular biochemistry, Vol. 368, Issue 1-2, pp. 137-45, (2012) (PubMed).

Wen, Zhou, Zhou, Zhou, Luo, Ma: "Change in hepatocyte growth factor concentration promote mesenchymal stem cell-mediated osteogenic regeneration." in: Journal of cellular and molecular medicine, Vol. 16, Issue 6, pp. 1260-73, (2012) (PubMed).

Wen, Jin, Zhou, Zhou, Luo, Ma: "HGF-transgenic MSCs can improve the effects of tissue self-repair in a rabbit model of traumatic osteonecrosis of the femoral head." in: PLoS ONE, Vol. 7, Issue 5, pp. e37503, (2012) (PubMed).

Ding, Sun, Zhen, Yu: "Urinary excretion and renal production of hepatocyte growth factor in children with Henoch-Schönlein purpura." in: The Journal of international medical research, Vol. 38, Issue 6, pp. 1933-41, (2011) (PubMed).

Lin, Xie, Chen, Zhang, Zeng, Qiang, Chen, Yang, Han, Zhang: "Treatment of experimental hepatic fibrosis by combinational delivery of urokinase-type plasminogen activator and hepatocyte growth factor genes." in: Liver international : official journal of the International Association for the Study of the Liver, Vol. 25, Issue 4, pp. 796-807, (2005) (PubMed).

Target Details for HGF

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: HGF (HGF Products)

Synonyms: DFNB39 antibody, F-TCF antibody, HGFB antibody, HPTA antibody, SF antibody, HGF antibody, C230052L06Rik antibody, HGF/SF antibody, NK1 antibody, NK2 antibody, SF/HGF antibody, hepatocyte growth factor antibody, HGF antibody, Hgf antibody

Background: Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenic factor. This gene is mapped to 7q21.11. It is secreted by mesenchymal cells and targets and acts primarily uponepithelial cells and endothelial cells, but also acts on haemopoietic progenitor cells. It has been shown to have a major role in embryonic organ development, specifically in myogenesis, in adult organ regeneration and in wound healing. HGF can regulate cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF also serves as a paracrine mediator to control placental development and growth. HGF was identified as one of the liver sinusoidal endothelial cell-derived paracrine mediators promoting hepatocyte growth.

Synonyms: DFNB39 antibody|F TCF antibody|Fibroblast derived tumor cytotoxic factor antibody|Hepatocyte growth factor (hepapoietin A, scatter factor) antibody|Hepatocyte growth factor antibody|Hepatocyte growth factor beta chain antibody|Hepatocyte growth factor precursor antibody|Hepatopoeitin-A antibody|Hepatopoietin A antibody|Hgf antibody|HGF_HUMAN antibody|HGFB antibody|HPTA antibody|Lung fibroblast derived mitogen antibody|OTTHUMP00000161349 antibody|OTTHUMP00000206710 antibody|OTTHUMP00000206711 antibody|OTTHUMP00000206712 antibody|OTTHUMP00000206713 antibody|OTTHUMP00000206730 antibody|Scatter factor antibody|SF antibody

Gene ID: 15234

UniProt: Q08048

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor