TGFA antibody (C-Term)

Catalog No. ABIN3042615

Product Details anti-TGFA Antibody

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Binding Specificity: AA 73-89, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TGFA antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Protransforming growth factor alpha(TGFA) detection. Tested with WB in Human.

Sequence: CHSGYVGARC EHADLLA

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Protransforming growth factor alpha(TGFA) detection. Tested with WB in Human.
Gene Name: transforming growth factor, alpha
Protein Name: Protransforming growth factor alpha

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human TGF alpha(73-89aa CHSGYVGARCEHADLLA), different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-TGFA antibody (ABIN3042615)

Li, Pan, Qiu: "Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage." in: International journal of clinical and experimental medicine, Vol. 7, Issue 12, pp. 5324-9, (2015) (PubMed).

Ruan, Xu, Li, Zhu, Pang, Wang, Cai, He, Yao, Ruan, Xu, Pan: "Induced autologous stem cell transplantation for treatment of rabbit renal interstitial fibrosis." in: PLoS ONE, Vol. 8, Issue 12, pp. e83507, (2013) (PubMed).

Zhang, Chen, Qi, Wang, Xiao, Zhu: "Inhibition of calcium-calmodulin-dependent kinase II suppresses cardiac fibroblast proliferation and extracellular matrix secretion." in: Journal of cardiovascular pharmacology, Vol. 55, Issue 1, pp. 96-105, (2010) (PubMed).

Target Details for TGFA

Target: TGFA (Transforming Growth Factor, alpha (TGFA))

Alternative Name: TGFA (TGFA Products)

Background: Transforming growth factor alpha(TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects . TGFalpha stimulates neural cell proliferation in the adult injured brain. TGFalpha was cited in the 2001 NIH Stem Cell report to the U.S. Congress as promising evidence for the ability of adult stem cells to restore function in neurodegenerative disorders.

Synonyms: EGF like TGF antibody|EGF-like TGF antibody|ETGF antibody|TFGA antibody|TGF A antibody|TGF type 1 antibody|TGF-alpha antibody|TGFA antibody|TGFA_HUMAN antibody|Transforming growth factor alpha antibody|Transforming growth factor alpha precursor antibody|Wa1 antibody|Waved 1 antibody

UniProt: P01135

Pathways: NF-kappaB Signaling, RTK Signaling, EGFR Signaling Pathway