Mip (AA 246-263), (C-Term) antibody

Catalog No. ABIN3042655

Product Details

Target: Mip

Binding Specificity: AA 246-263, C-Term

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Lens fiber major intrinsic protein(MIP) detection. Tested with WB in Human,Mouse,Rat.

Sequence: NGQPEGTGEP VELKTQAL

Cross-Reactivity (Details): Predicted Cross Reactivity: human, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Lens fiber major intrinsic protein(MIP) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: major intrinsic protein of lens fiber
Protein Name: Lens fiber major intrinsic protein

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Aquaporin 0(246-263aa NGQPEGTGEPVELKTQAL), identical to the related rat sequence, and different from the related human sequence by two amino acids.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Predicted Species: Human, Rat, The detection limit for MIP is approximately 1.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References

Liu, An, Liu, Li, Tang, Chen: "Mouse lung slices: An ex vivo model for the evaluation of antiviral and anti-inflammatory agents against influenza viruses." in: Antiviral research, Vol. 120, pp. 101-11, (2015) (PubMed).

Sun, Zhao, Yao, Zhao, Wu, Wang, Pan, Miao, Guo, Lu: "Wogonoside protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting NF-?B and NLRP3 inflammasome activation." in: Biochemical pharmacology, Vol. 94, Issue 2, pp. 142-54, (2015) (PubMed).

Bruns, Cadeddu, Brueckmann, Fröbel, Geyh, Büst, Fischer, Roels, Wilk, Schildberg, Hünerlitürkoglu, Zilkens, Jäger, Steidl, Zohren, Fenk, Kobbe, Brors, Czibere, Schroeder, Trumpp, Haas: "Multiple myeloma-related deregulation of bone marrow-derived CD34(+) hematopoietic stem and progenitor cells." in: Blood, Vol. 120, Issue 13, pp. 2620-30, (2012) (PubMed).

Target Details

Target: Mip

Synonyms: Aqp0 antibody, Cat antibody, Hfi antibody, Lop antibody, MIP26 antibody, Svl antibody, shrivelled antibody, major intrinsic protein of lens fiber antibody, Mip antibody

Background: Lens fiber major intrinsic protein also called MIP26 or MP26 is a protein that in humans is encoded by the MIP gene. MIP is a member of the water-transporting aquaporins as well as the original member of the MIP family of channel proteins. Using 2-color fluorescence in situ hybridization on high-resolution R-banded chromosomes and human genomic DNA clones for MIP as probes, this gene was found that located in close proximity in region 12q13. MIP plays a crucial role in the development of a transparent eye lens. This gene may be responsible for regulating the osmolarity of the lens and interactions between homotetramers from adjoining membranes may stabilize cell junctions in the eye lens core.

Synonyms: AQP 0 antibody|AQP0 antibody|Aquaporin antibody|Aquaporin0 antibody|Lens fiber major intrinsic protein antibody|LIM 1 antibody|LIM1 antibody|Major intrinsic protein 26kD antibody|Major intrinsic protein of lens fiber antibody|MIP 26 antibody|MIP antibody|MIP26 antibody|MP 26 antibody|MP26 antibody|PALM antibody

UniProt: P51180