Caspase 12 antibody (N-Term)
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- Target See all Caspase 12 (CASP12) Antibodies
- Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))
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Binding Specificity
- AA 71-84, N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Caspase 12 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Rabbit IgG polyclonal antibody for Inactive caspase-12(CASP12) detection. Tested with WB, IHC-P in Human.
- Sequence
- KIFREHLWNS KKQL
- Cross-Reactivity (Details)
- No cross reactivity with other proteins.
- Characteristics
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Rabbit IgG polyclonal antibody for Inactive caspase-12(CASP12) detection. Tested with WB, IHC-P in Human.
Gene Name: caspase 12(gene/pseudogene)
Protein Name: Inactive caspase-12 - Purification
- Immunogen affinity purified.
- Immunogen
- A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-12(71-84aa KIFREHLWNSKKQL).
- Isotype
- IgG
- Top Product
- Discover our top product CASP12 Primary Antibody
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- Application Notes
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for CASP12 is approximately 0.5 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - Comment
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Concentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- Preservative
- Thimerosal (Merthiolate), Sodium azide
- Precaution of Use
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - Expiry Date
- 12 months
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Atmospheric pressure room temperature plasma jets facilitate oxidative and nitrative stress and lead to endoplasmic reticulum stress dependent apoptosis in HepG2 cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e73665, (2013) (PubMed).
: "Study of histopathological and molecular changes of rat kidney under simulated weightlessness and resistance training protective effect." in: PLoS ONE, Vol. 6, Issue 5, pp. e20008, (2011) (PubMed).
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Atmospheric pressure room temperature plasma jets facilitate oxidative and nitrative stress and lead to endoplasmic reticulum stress dependent apoptosis in HepG2 cells." in: PLoS ONE, Vol. 8, Issue 8, pp. e73665, (2013) (PubMed).
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- Target
- Caspase 12 (CASP12) (Caspase 12 (Gene/pseudogene) (CASP12))
- Alternative Name
- CASP12 (CASP12 Products)
- Synonyms
- Caspase-12 antibody, CASP-12 antibody, CASP12P1 antibody, caspase 12 antibody, caspase 12 (gene/pseudogene) antibody, Casp12 antibody, CASP12 antibody
- Background
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Caspase 12 is an enzyme known as a cysteine protease. It belongs to a family of enzymes called caspases that cleave their substrates at C-terminal aspartic acid residues. The CASP12 gene was mapped to chromosome 11q22.3. Csp12L attenuates the inflammatory and innate immune response to endotoxins and in doing so may constitute a risk factor for developing sepsis. The estrogen-mediated inhibition of CSP12L expression is a built-in mechanism that has evolved to protect females from infection by favoring a more robust inflammatory response to pathogens, perhaps notably in childbirth.
Synonyms: Apoptosis related cysteine protease antibody|CASP 12 antibody|CASP-12 antibody|casp12 antibody|CASP12P1 antibody|caspase 12(gene/pseudogene) antibody|caspase 12 pseudogene 1 antibody|Caspase 12 psuedogene 1 antibody|Caspase12 antibody|CASPC_HUMAN antibody|Inactive caspase-12 antibody|OTTHUMP00000207032 antibody|UNQ9415 antibody - Pathways
- Apoptosis, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response
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