CDK2 antibody (C-Term)

Catalog No. ABIN3042680

Product Details anti-CDK2 Antibody

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Binding Specificity: AA 277-298, C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK2 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB in Human,Mouse,Rat.

Sequence: AKAALAHPFF QDVTKPVPHL RL

Cross-Reactivity (Details): Predicted Cross Reactivity: mouse, rat
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

Characteristics: Rabbit IgG polyclonal antibody for Cyclin-dependent kinase 2(CDK2) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: cyclin-dependent kinase 2
Protein Name: Cyclin-dependent kinase 2

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk2(277-298aa AKAALAHPFFQDVTKPVPHLRL), identical to the related rat and mouse sequences.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Preservative: Thimerosal (Merthiolate), Sodium azide

Precaution of Use: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Expiry Date: 12 months

References for anti-CDK2 antibody (ABIN3042680)

Ren, Qiu, Lü, Ru, Li, Xiang, Yu, Zhang: "TALENs-directed knockout of the full-length transcription factor Nrf1α that represses malignant behaviour of human hepatocellular carcinoma (HepG2) cells." in: Scientific reports, Vol. 6, pp. 23775, (2017) (PubMed).

Han, Ge, Zhang, Zhou, Ji, Yang, Zhang: "Effects of tripterygium glycosides on restenosis following endovascular treatment." in: Molecular medicine reports, Vol. 13, Issue 6, pp. 4959-68, (2017) (PubMed).

Yang, Khan, He, Yao, Li, Gao, Ma: "Tubeimoside-1 induces oxidative stress-mediated apoptosis and G0/G1 phase arrest in human prostate carcinoma cells in vitro." in: Acta pharmacologica Sinica, Vol. 37, Issue 7, pp. 950-62, (2017) (PubMed).

Zhong, Zhang, Wang, Wang, Song, Zhang, Yu: "Rheum emodin inhibits enterovirus 71 viral replication and affects the host cell cycle environment." in: Acta pharmacologica Sinica, Vol. 38, Issue 3, pp. 392-401, (2017) (PubMed).

Ren, Huang, Xu, Yang, Yang, Hu: "Isoflavone lupiwighteone induces cytotoxic, apoptotic, and antiangiogenic activities in DU-145 prostate cancer cells." in: Anti-cancer drugs, Vol. 26, Issue 6, pp. 599-611, (2015) (PubMed).

Zhou, Lu, Liu, Guo, Liu, Zhou, Yang, Mi, Xu: "Platycodin D induces tumor growth arrest by activating FOXO3a expression in prostate cancer in vitro and in vivo." in: Current cancer drug targets, Vol. 14, Issue 9, pp. 860-71, (2015) (PubMed).

Yu, Zhang, Ren, Zhong, Li, Wang, Li, Liu, Zhao, Zhang, Yu: "Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D." in: Cell cycle (Georgetown, Tex.), Vol. 14, Issue 3, pp. 425-36, (2015) (PubMed).

Zhao, Li, Gao, Wang, Yan, Zhan, Liu, Mao, Chen, Wang et al.: "Design, synthesis and biological evaluation of N-alkyl or aryl substituted isoindigo derivatives as potential dual cyclin-dependent kinase 2 (CDK2)/glycogen synthase kinase 3? (GSK-3?) ..." in: European journal of medicinal chemistry, Vol. 86, pp. 165-74, (2014) (PubMed).

Cheng, Chen, Wang, Ding, Shi: "The effects of urokinase-type plasminogen activator (uPA) on cell proliferation and phenotypic transformation of rat mesangial cells induced by high glucose." in: Diabetes research and clinical practice, Vol. 103, Issue 3, pp. 489-95, (2014) (PubMed).

Ye, Chen, Chen, Xie, Yang, Lou, Yu: "XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line." in: Chemico-biological interactions, Vol. 183, Issue 1, pp. 133-41, (2009) (PubMed).

Target Details for CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Alternative Name: CDK2 (CDK2 Products)

Synonyms: p33(CDK2) antibody, A630093N05Rik antibody, wu:fa10c02 antibody, zgc:56598 antibody, zgc:77684 antibody, eg1 antibody, CDKN2 antibody, cdk2L antibody, cyclin dependent kinase 2 antibody, cyclin-dependent kinase 2 antibody, cyclin-dependent kinase 2 S homeolog antibody, CDK2 antibody, Cdk2 antibody, cdk2 antibody, cdk2.S antibody

Background: CDK2, Cyclin-Dependent Kinase2, is also know as P33. The CDK2 protein was highly homologous to p34(CDC2) kinase and more significantly homologous to Xenopus Eg1 kinase, suggesting that CDK2 is the human homolog of Eg1. The CDK2 gene is mapped to 12q13, the same region to which the CDK4 gene maps. Human cyclin A binds independently to 2 kinases, p34(cdc2) or p33. In adenovirus-transformed cells, the viral E1A oncoprotein seems to associate with p33/cyclin A but not with p34(cdc2)/cyclin A. The gene for p33 shares 65 % sequence identity with p34(cdc2). P33(cdk2) plays a unique role in cell cycle regulation of vertebrate cells.

Synonyms: Cdc2 related protein kinase antibody|cdc2-related protein kinase antibody|Cdk 2 antibody|CDK2 antibody|CDK2_HUMAN antibody|Cell devision kinase 2 antibody|Cell division kinase 2 antibody|Cell division protein kinase 2 antibody|Cyclin dependent kinase 2 antibody|cyclin dependent kinase 2-alpha antibody|Cyclin-dependent kinase 2 antibody|p33 protein kinase antibody|p33(CDK2) antibody

UniProt: P24941

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA