COL4A1 antibody (AA 1445-1669)

Catalog No. ABIN3042755

Product Details anti-COL4A1 Antibody

Target: COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))

Binding Specificity: AA 1445-1669

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COL4A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Collagen alpha-1(IV) chain(COL4A1) detection. Tested with WB, IHC-P in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Collagen alpha-1(IV) chain(COL4A1) detection. Tested with WB, IHC-P in Human.
Gene Name: collagen, type IV, alpha 1
Protein Name: Collagen alpha-1(IV) chain

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Collagen IV is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-COL4A1 antibody (ABIN3042755)

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Zhang, Wang, Cui, Zhang, Liu, Chu, Liu, Zhang, Chu: "Continuing treatment with Salvia miltiorrhiza injection attenuates myocardial fibrosis in chronic iron-overloaded mice." in: PLoS ONE, Vol. 10, Issue 4, pp. e0124061, (2016) (PubMed).

Su, Wei, Wei, Liu, Li: "Effects of rhBNP on myocardial fibrosis after myocardial infarction in rats." in: International journal of clinical and experimental pathology, Vol. 8, Issue 6, pp. 6407-15, (2016) (PubMed).

Yao, Liu, Li, Hao: "Silica promotes the transdifferentiation of rat circulating fibrocytes in vitro." in: Molecular medicine reports, Vol. 12, Issue 4, pp. 5828-36, (2016) (PubMed).

Shan, Zhou, Xia, Qian: "Astragalus membranaceus ameliorates renal interstitial fibrosis by inhibiting tubular epithelial-mesenchymal transition in vivo and in vitro." in: Experimental and therapeutic medicine, Vol. 11, Issue 5, pp. 1611-1616, (2016) (PubMed).

Zhao, Liu, Shi, Xie, Cui, Li, Li, Ren: "Role of rat autologous skin fibroblasts and mechanism underlying the repair of depressed scars." in: Experimental and therapeutic medicine, Vol. 12, Issue 2, pp. 945-950, (2016) (PubMed).

Zhang, Teng, Liu, Zhang, Liu: "Gene expression profile analyze the molecular mechanism of CXCR7 regulating papillary thyroid carcinoma growth and metastasis." in: Journal of experimental & clinical cancer research : CR, Vol. 34, pp. 16, (2015) (PubMed).

Luo, Deng, Lamsal, Xu, Xiang, Cheng: "AMP-activated protein kinase alleviates extracellular matrix accumulation in high glucose-induced renal fibroblasts through mTOR signaling pathway." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 35, Issue 1, pp. 191-200, (2015) (PubMed).

Qiu, Zhang, Zhao, Zhang, Wu, Guo, Hu: "Enhancement of endothelial differentiation of adipose derived mesenchymal stem cells by a three-dimensional culture system of microwell." in: Biomaterials, Vol. 53, pp. 600-8, (2015) (PubMed).

Yan, Zhang, Kolkin, Wang, Xia, Fan: "Mechanisms of nerve capping technique in prevention of painful neuroma formation." in: PLoS ONE, Vol. 9, Issue 4, pp. e93973, (2015) (PubMed).

Xu, Zhang, Xu, Guo, Wang, Wu, Wang, Luo, Zhou: "Antiphotoaging effect of conditioned medium of dedifferentiated adipocytes on skin in vivo and in vitro: a mechanistic study." in: Stem cells and development, Vol. 24, Issue 9, pp. 1096-111, (2015) (PubMed).

Yang, Ni, Duan, Wang, Chen, Chen, Chaugai, Zeldin, Tang, Wang: "CYP epoxygenase 2J2 prevents cardiac fibrosis by suppression of transmission of pro-inflammation from cardiomyocytes to macrophages." in: Prostaglandins & other lipid mediators, Vol. 116-117, pp. 64-75, (2015) (PubMed).

Ye, Wang, Zhang, Song, Yang, Li, Li, Su, Yang, Ma: "Over-expression of calpastatin inhibits calpain activation and attenuates post-infarction myocardial remodeling." in: PLoS ONE, Vol. 10, Issue 3, pp. e0120178, (2015) (PubMed).

Li, Kong, Ma, Gao, Ma: "Uighur medicine abnormal savda munzip (ASMq) suppresses expression of collagen and TGF-β1 with concomitant induce Smad7 in human hypertrophic scar fibroblasts." in: International journal of clinical and experimental medicine, Vol. 8, Issue 6, pp. 8551-60, (2015) (PubMed).

Liu, Lu, Li, Wei, Li: "Engineering blood vessels through micropatterned co-culture of vascular endothelial and smooth muscle cells on bilayered electrospun fibrous mats with pDNA inoculation." in: Acta biomaterialia, Vol. 11, pp. 114-25, (2014) (PubMed).

Ji, Wu, Ma, Ma, Qin: "The effect of resveratrol on the expression of AdipoR1 in kidneys of diabetic nephropathy." in: Molecular biology reports, Vol. 41, Issue 4, pp. 2151-9, (2014) (PubMed).

Wenbin, Guojun: "Resveratrol Ameliorates Diabetes-induced Renal Damage through Regulating the Expression of TGF-?1, Collagen IV and Th17/Treg-related Cytokines in Rats." in: The West Indian medical journal, Vol. 63, Issue 1, pp. 20-5, (2014) (PubMed).

Target Details for COL4A1

Target: COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))

Alternative Name: COL4A1 (COL4A1 Products)

Background: COL4A1, also known as ICH or Collagen alpha-1(IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Synonyms: Arresten antibody|Canstatin antibody|CO4A1_HUMAN antibody|COL4A1 antibody|COL4A1 NC1 domain antibody|COL4A2 antibody|COL4A3 antibody|COL4A4 antibody|COL4A5 antibody|Collagen Alpha 1(IV) Chain antibody|Collagen Alpha 2(IV) Chain antibody|collagen alpha-1(IV) chain antibody|Collagen IV Alpha 1 Polypeptide antibody|Collagen IV Alpha 2 Polypeptide antibody|Collagen Of Basement Membrane Alpha 1 Chain antibody|Collagen Of Basement Membrane Alpha 2 Chain antibody|Collagen Type IV Alpha 1 antibody|Collagen Type IV Alpha 2 antibody|Collagen Type IV Alpha 3 antibody|Collagen Type IV Alpha 4 antibody|Collagen Type IV Alpha 5 antibody|DKFZp686I14213 antibody|FLJ22259 antibody

Gene ID: 1282

UniProt: P02462

Pathways: Skeletal Muscle Fiber Development, Growth Factor Binding